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| Status |
Public on Aug 30, 2023 |
| Title |
Histone divergence in Trypanosoma brucei results in unique alterations in nucleosome structure |
| Organism |
synthetic construct |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Eukaryotes have an array of diverse mechanisms for organising and using their genomes, but the histones that make up chromatin are highly conserved. Unusually, histones from Kinetoplastids are highly divergent. The structural and functional consequences of this variation are unknown. Here, we have biochemically characterised nucleosome core particles (NCPs) from the Kinetoplastid parasite Trypanosoma brucei. A structure of the T. brucei NCP reveals that global histone architecture is conserved, but specific sequence alterations lead to distinct DNA and protein interaction interfaces. The T. brucei NCP is unstable and has weakened DNA binding overall. However, dramatic changes at the H2A-H2B interface introduce local reinforcement of DNA contacts. The T. brucei acidic patch has altered topology and is refractory to known binders, indicating that the nature of chromatin interactions in T. brucei may be unique. Overall, our results provide a detailed molecular basis for understanding evolutionary divergence in chromatin structure.
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| Overall design |
Sequencing fragments of Widom 601 145 bp DNA produced from limited miccrococcal nuclease digestion of Homo sapiens and Trypanosoma brucei nucleosome core particles
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| Contributor(s) |
Deak G, Wapenaar H, Sandoval G, Chen R, Taylor MR, Burdett H, Watson JA, Tuijtel MW, Webb S, Wilson MD |
| Citation(s) |
37427792 |
| Submission date |
Feb 24, 2023 |
| Last update date |
Aug 30, 2023 |
| Contact name |
Shaun Michael Webb |
| E-mail(s) |
shaun.webb@ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
Wellcome Trust Centre for Cell Biology
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| Lab |
Bioinformatics Core Facility
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| Street address |
2.21 Swann Building, Kings Buildings
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| City |
Edinburgh |
| ZIP/Postal code |
EH9 3JR |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL17769 |
Illumina MiSeq (synthetic construct) |
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| Samples (2) |
| GSM7061750 |
Homo sapiens MNase digestion products |
| GSM7061751 |
Trypanosoma brucei MNase digestion products |
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| Relations |
| BioProject |
PRJNA938394 |
