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| Status |
Public on May 28, 2023 |
| Title |
Intranasal delivery of NS1-deleted influenza virus vectored COVID-19 vaccine restrains the SARS-CoV-2 inflammatory response (Mouse Lung RNA-seq Data) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The emergence of SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) variants and “anatomical escape” characteristics threaten the effectiveness of current coronavirus disease (COVID-19) vaccines. There is an urgent need to understand the immunological mechanism of broad-spectrum respiratory tract protection to guide broader vaccines development. In this study, we investigated immune responses induced by an NS1-deleted influenza virus vectored intranasal COVID-19 vaccine (dNS1-RBD) which provides broad-spectrum protection against SARS-CoV-2 variants. Intranasal delivery of dNS1-RBD induced innate immunity, trained immunity and tissue-resident memory T cells covering the upper and lower respiratory tract. It restrained the inflammatory response by suppressing early phase viral load post SARS-CoV-2 challenge and attenuating pro-inflammatory cytokine (IL-6, IL-1B, and IFN-γ) levels, thereby reducing excess immune-induced tissue injury compared with the control group. By inducing local cellular immunity and trained immunity, intranasal delivery of NS1-deleted influenza virus vectored vaccine represents a broad-spectrum COVID-19 vaccine strategy to reduce disease burden.
To investigate the immune response generated by dNS1-RBD vaccine in C57BL/6 mouse lung, we vaccinated C57BL/6 mice and collect the lung samples to sequence for the RNA-seq data, then performed gene expression analysis using data obtained from RNA-seq of 5 different time points before and after vaccinated with dNS1-RBD vaccine.
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| Overall design |
Comparative gene expression analysis and enrichment analysis analysis of lung RNA-seq data for non-vaccinated and different time points(1,7,14,28 days post vaccinated)
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| Contributor(s) |
Zhang L, Jiang Y, He J |
| Citation(s) |
37433761 |
| |
| Submission date |
Mar 19, 2023 |
| Last update date |
Sep 12, 2023 |
| Contact name |
Jinhang He |
| E-mail(s) |
hejinhang@stu.xmu.edu.cn
|
| Phone |
15821251893
|
| Organization name |
Xiamen University
|
| Street address |
No.4221, South Xiang'an Road
|
| City |
Xiamen |
| State/province |
Fujian |
| ZIP/Postal code |
361104 |
| Country |
China |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE227649 |
Intranasal delivery of NS1-deleted influenza virus vectored COVID-19 vaccine restrains the SARS-CoV-2 inflammatory response |
|
| Relations |
| BioProject |
PRJNA946278 |
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