 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 28, 2023 |
| Title |
vaccine_day28_2 |
| Sample type |
SRA |
| |
|
| Source name |
C57BL/6 Lung, 28th day post vaccinated with dNS1-RBD vaccine
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Lung
strain: C57BL/6
treatment: Vaccinated with dNS1-RBD Vaccine
time: Day 28
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The mouse lung lobe was removed, shredded into small pieces and stored in RNA Later Solution (Thermo Fisher Scientific) for a maximum of 24 h at 4 °C. Lung tissue was homogenized using TissueLyser II (Qiagen, Hilden, Germany) and RNA extraction was performed according to the manufacturer’s instructions (QIAamp Viral RNA Mini Kit (Qiagen)).
Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer’s protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number (RIN) ≥ 7 were subjected to the subsequent analysis. The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Sequence reads were trimmed and adaptor removed by using fastp.
Cleanned sequence reads were mapped to GRcm39 Hisat2
Raw count were calculated by using featurecounts
Assembly: GRcm39
Supplementary files format and content: tab-delimited txt files include raw count values for each Sample
|
| |
|
| Submission date |
Mar 19, 2023 |
| Last update date |
May 28, 2023 |
| Contact name |
Jinhang He |
| E-mail(s) |
hejinhang@stu.xmu.edu.cn
|
| Phone |
15821251893
|
| Organization name |
Xiamen University
|
| Street address |
No.4221, South Xiang'an Road
|
| City |
Xiamen |
| State/province |
Fujian |
| ZIP/Postal code |
361104 |
| Country |
China |
| |
|
| Platform ID |
GPL17021 |
| Series (2) |
| GSE227647 |
Intranasal delivery of NS1-deleted influenza virus vectored COVID-19 vaccine restrains the SARS-CoV-2 inflammatory response (Mouse Lung RNA-seq Data) |
| GSE227649 |
Intranasal delivery of NS1-deleted influenza virus vectored COVID-19 vaccine restrains the SARS-CoV-2 inflammatory response |
|
| Relations |
| BioSample |
SAMN33818979 |
| SRA |
SRX19719240 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
