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Series GSE227757 Query DataSets for GSE227757
Status Public on May 09, 2023
Title Context dependent function of transcriptional regulator Rap1 in gene silencing and activation [ChIPseq I]
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary As part of a study on the role of Rap1 at the promoters of HML and MAT in S cerevisiae, we endogenously tagged Rap1 with a 3xV5 tag and performed ChIPseq in a variety of genetic backgrounds including: silenced (SIR); unsilenced (sir1∆/sir3∆/sir4∆); in strains witha wild type alpha2 promoter (wtpromoter); or with a point mutation at the promoter (mutpromoter).
 
Overall design 3xV5-Rap1 ChIPseq. Replicates are included for every sample.
 
Contributor(s) Bondra ER, Rine JD
Citation(s) 37214837, 37769255
Submission date Mar 20, 2023
Last update date Oct 18, 2023
Contact name Eliana Rose Bondra
Organization name University of California, Berkeley
Department Molecular and Cell Biology
Lab Rine
Street address 440 Barker Hall
City Berkeley
State/province California
ZIP/Postal code 94720
Country USA
 
Platforms (1)
GPL27812 Illumina NovaSeq 6000 (Saccharomyces cerevisiae)
Samples (42)
GSM7107596 SIR_wtpromoter_V5Rap1 ChIP rep1
GSM7107597 SIR_wtpromoter_V5Rap1 input rep1
GSM7107598 SIR_wtpromoter_V5Rap1 ChIP rep2
This SubSeries is part of SuperSeries:
GSE227763 Context dependent function of transcriptional regulator Rap1 in gene silencing and activation
Relations
BioProject PRJNA946776

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE227757_RAW.tar 2.6 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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