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| Status |
Public on Jan 01, 2016 |
| Title |
Transcriptional analysis of AHLs signals add in experiment inYersinia pestis CO92 at 37°C |
| Organism |
Yersinia pestis |
| Experiment type |
Expression profiling by array
|
| Summary |
Yersinia pestis, the etiological agent of plague, is able to sense cell density by quorum sensing. The function of quorum sensing in Y. pestis is not clear. Here, the process of AHL quorum sensing was investigated by comparing transcript profiles when two AHL quorum-sensing signals are added in. The strain ∆pgm (pigmentation-negative) mutant R88 was called wild type. The two AHLs signals are N-(3-Oxooctanoyl)-L-homoserine lactone and N-Hexanoyl-DL-homoserine lactone.The control consisted of cells grown and treated under the same conditions without added signals.
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| Overall design |
Six independent RNA samples from R88 cultures were paired with six independent RNA samples from two AHLs added cultures for hybridization to six two-color microarrays. A dye-swap design was used to remove the Cy5 and Cy3 dye bias.
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| Contributor(s) |
Minion C, Yu J |
| Citation(s) |
23959719 |
| Submission date |
Jul 09, 2010 |
| Last update date |
Apr 01, 2016 |
| Contact name |
Chris Minion |
| E-mail(s) |
fcminion@iastate.edu
|
| Phone |
515-294-6347
|
| Fax |
515-294-8500
|
| Organization name |
Iowa State University
|
| Department |
VMPM
|
| Street address |
1130 Vet Med
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10439 |
WUSTL Yersinia pestis 14K array |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE22850 |
Outlier detection of biologically significant genes from combinatorial microarray data |
| GSE30373 |
Global gene expression analysis of *Yersinia pestis* AHL quorum sensing |
|
| Relations |
| BioProject |
PRJNA129127 |
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