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| Status |
Public on Sep 05, 2013 |
| Title |
Transcription analysis of AI-2 signal add in experiment in Yersinia pestis CO92 at 37°C |
| Organism |
Yersinia pestis |
| Experiment type |
Expression profiling by array
|
| Summary |
Yersinia pestis, the etiological agent of plague, is able to sense cell density by quorum sensing. The function of quorum sensing in Y. pestis is not clear. Here, the process of autoinducer-2 (AI-2) quorum sensing was investigated by comparing transcript profiles when AI-2 quorum-sensing signal is added in. The strain ∆pgm(pigmentation-negative) mutant R88 was used as wild type. The control consisted of cells grown and treated under the same conditions without added signals.
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| Overall design |
Six independent RNA samples from Y. pestis CO92 ∆pgm cultures were paired with six independent RNA samples from AI-2 signal added cultures for hybridization to six two-color microarrays. A dye-swap design was used to remove the Cy5 and Cy3 dye bias.
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| Contributor(s) |
Minion C, Yu J |
| Citation(s) |
23959719 |
| |
| Submission date |
Jul 09, 2010 |
| Last update date |
Sep 07, 2013 |
| Contact name |
Chris Minion |
| E-mail(s) |
fcminion@iastate.edu
|
| Phone |
515-294-6347
|
| Fax |
515-294-8500
|
| Organization name |
Iowa State University
|
| Department |
VMPM
|
| Street address |
1130 Vet Med
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10439 |
WUSTL Yersinia pestis 14K array |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE22850 |
Outlier detection of biologically significant genes from combinatorial microarray data |
| GSE30342 |
AI-2 quorum sensing in *Yersinia pestis* |
|
| Relations |
| BioProject |
PRJNA129129 |
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