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Series GSE228554 Query DataSets for GSE228554
Status Public on Oct 01, 2023
Title Sexually dimorphic impact of constitutive androstane receptor activation in mouse liver
Organism Mus musculus
Experiment type Expression profiling by array
Summary The constitutive androstane receptor (CAR) is a nuclear receptor able to recognize a large panel of drugs leading to the modulation of the expression of its target genes involved in xenobiotic detoxication and energy metabolism. CAR hepatic activity is thought to be higher in women than in men, but its target genes and metabolic role in female mice have never been thoroughly studied. Here, we investigated the liver gene expression in Car+/+ vs Car-/- male and female C57Bl6/J mice treated with the CAR-specific agonist, 1,4-bis[2-(3,5-dichloropyridyloxy)] benzene (TPOBOP), or with Corn Oil
Overall design Car+/+ and Car-/- littermate mice were separated by sex and genotype at 4 weeks and randomly allocated to the different experimental groups: Male Car+/+ Corn Oil (M Car+/+ CO, n=6), Male Car+/+ TCPOBOP (M Car+/+ TCPOBOP, n=6), Male Car-/- Corn Oil (M Car-/- CO, n=6), Male Car-/- TCPOBOP (M Car-/- TCPOBOP, n=6), Female Car+/+ Corn Oil (F Car+/+ CO, n=6), Female Car+/+ TCPOBOP (F Car+/+ TCPOBOP, n=6), Female Car-/- Corn Oil (F Car-/- CO, n=6), Female Car-/- TCPOBOP (F Car-/- TCPOBOP, n=6) (one cage per group). Mice included in TCPOBOP groups received a daily intraperitoneal injection of 1,4-bis[2-(3,5-dichloro pyridyloxy)]Benzene (TCPOBOP, Sigma Aldrich) diluted in corn oil at 3mg/kg for 4 days while CO mice received corn oil only (Sigma Aldrich). At ZT16 (6 hours after the last TCPOBOP injection), mice were anesthetized with Isoflurane and Xylasine (2%, 2mg/kg) and liver samples were collected.
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Contributor(s) Ellero-Simatos S, Naylies C, Lippi Y
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Submission date Mar 30, 2023
Last update date Oct 16, 2023
Contact name Sandrine Ellero-Simatos
Organization name INRAe
Street address 180 chemin de Tournefeuille
City Toulouse
ZIP/Postal code 31300
Country France
Platforms (1)
GPL21163 Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version]
Samples (48)
GSM7123963 Liver - Male_CARWT_Corn.Oil - Rep1
GSM7123964 Liver - Male_CARWT_TCPOBOP - Rep1
GSM7123965 Liver - Male_CARKO_Corn.Oil - Rep3
BioProject PRJNA950344

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE228554_RAW.tar 606.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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