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Series GSE228877 Query DataSets for GSE228877
Status Public on Jun 13, 2023
Title RIF1 regulates replication origin activity and early replication timing in B cells [PRO-seq]
Organism Mus musculus
Experiment type Other
Summary The mammalian DNA replication timing (RT) program is crucial for the proper functioning and integrity of the genome. The best-known mechanism for controlling RT is the suppression of late origins of replication in heterochromatin by RIF1. Here, we report that in antigen-activated, hypermutating B lymphocytes, RIF1 binds predominantly to early-replicating active chromatin, regulates early origin firing and promotes early replication. However, RIF1 has a minor role in gene expression and genome organization in B cells. Furthermore, we find that RIF1 functions in a complementary and non-epistatic manner with minichromosome maintenance (MCM) proteins to establish early RT signatures genome-wide and, specifically, to ensure the early replication of highly transcribed genes. These findings reveal a new layer of regulation within the B cell RT program, driven by the coordinated activity of RIF1 and MCM proteins.
Overall design PRO-seq of priB and CH12 WT cells
Web link
Contributor(s) Malzl D, Peycheva M, Rahjouei A, Gnan S, Klein KN, Nazarova M, Schoeberl UE, Gilbert DM, Buonomo SC, Di Virgilio M, Neumann T, Pavri R
Citation(s) 38081811
Submission date Apr 03, 2023
Last update date Dec 14, 2023
Contact name Tobias Neumann
Organization name IMP
Street address Campus-Vienna-Biocenter 1
City Vienna
ZIP/Postal code 1030
Country Austria
Platforms (1)
GPL21626 NextSeq 550 (Mus musculus)
Samples (4)
GSM7140988 CH12_PROseq_shLacZ_rep1
GSM7140989 CH12_PROseq_shLacZ_rep2
GSM7140990 priB_PROseq_WT_rep1
This SubSeries is part of SuperSeries:
GSE228880 RIF1 regulates early replication timing in murine B cells.
BioProject PRJNA951773

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Supplementary file Size Download File type/resource 739.7 Kb (ftp)(http) BW 826.5 Kb (ftp)(http) BW
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