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| Status |
Public on Jun 13, 2023 |
| Title |
RIF1 regulates replication origin activity and early replication timing in B cells [PRO-seq] |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
The mammalian DNA replication timing (RT) program is crucial for the proper functioning and integrity of the genome. The best-known mechanism for controlling RT is the suppression of late origins of replication in heterochromatin by RIF1. Here, we report that in antigen-activated, hypermutating B lymphocytes, RIF1 binds predominantly to early-replicating active chromatin, regulates early origin firing and promotes early replication. However, RIF1 has a minor role in gene expression and genome organization in B cells. Furthermore, we find that RIF1 functions in a complementary and non-epistatic manner with minichromosome maintenance (MCM) proteins to establish early RT signatures genome-wide and, specifically, to ensure the early replication of highly transcribed genes. These findings reveal a new layer of regulation within the B cell RT program, driven by the coordinated activity of RIF1 and MCM proteins.
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| Overall design |
PRO-seq of priB and CH12 WT cells
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| Web link |
https://www.biorxiv.org/content/10.1101/2023.03.31.535086v2.full
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| Contributor(s) |
Malzl D, Peycheva M, Rahjouei A, Gnan S, Klein KN, Nazarova M, Schoeberl UE, Gilbert DM, Buonomo SC, Di Virgilio M, Neumann T, Pavri R |
| Citation(s) |
38081811 |
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| Submission date |
Apr 03, 2023 |
| Last update date |
Dec 14, 2023 |
| Contact name |
Tobias Neumann |
| Organization name |
IMP
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| Street address |
Campus-Vienna-Biocenter 1
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| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
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| Platforms (1) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE228880 |
RIF1 regulates early replication timing in murine B cells. |
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| Relations |
| BioProject |
PRJNA951773 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE228877_CH12_PROseq_shLacZ_20kb.bw |
739.7 Kb |
(ftp)(http) |
BW |
| GSE228877_priB_PROseq_WT_20kb.bw |
826.5 Kb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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