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| Status |
Public on Oct 20, 2023 |
| Title |
Gene expression in dormant, germinating and thermoinhibited Arabidopsis seeds |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
To establish the basis for understanding molecular mechanism of seed germination response to temperature, we analyzed transcriptomes in freshly harvested dormant and dry stored after-ripened seeds. The after-ripened seeds started to show visible germination from 36h after the start of imbibition, and almost all the seeds germinated after 3 days. The freshly harvested seeds stayed dormant by imbibition at 26°C, and germination of the after-ripened seeds was almost completely inhibited at 34°C. Total RNA was prepared from 0 (dry), 6 and 24h imbibed seeds to find regulatory genes of seed dormancy and germination.
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| Overall design |
Transcriptomes in Arabidopsis thaliana Col-0 dormant seeds (imbibed at 26°C ) and after-ripened seeds (imbibed at 26°C and 34°C) were analyzed at 0, 6 and 24 hours after imbibition. Three independent experiments were performed at each time point using different seed batches for each experiment.
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| Contributor(s) |
Tachibana N, Sano T, Nakazawa Y, Koshimizu S, Kawakami N |
| Citation missing |
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| |
| Submission date |
Apr 07, 2023 |
| Last update date |
Oct 20, 2023 |
| Contact name |
Naoto Kawakami |
| E-mail(s) |
kawakami@meiji.ac.jp
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| Phone |
+81 44 934 7821
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| Organization name |
Meiji University
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| Department |
Department of Life Sciences
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| Lab |
Laboratory of Plant Molecular Physiology
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| Street address |
1-1-1 Higashimita, Tama-ku
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| City |
Kawasaki |
| State/province |
Kanagawa |
| ZIP/Postal code |
214-8571 |
| Country |
Japan |
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| Platforms (1) |
| GPL9020 |
Agilent-021169 Arabidopsis 4 Oligo Microarray (V4) (Feature Number version) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA953376 |
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