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| Status |
Public on Jun 01, 2023 |
| Title |
JANUS, a spliceosome-associated protein, promotes miRNA biogenesis in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
MicroRNAs (miRNAs) are important regulators of gene expression. Their levels are precisely controlled through modulating the activity of the microprocesser complex (MC). Here we report that JANUS, a homology of the conserved U2 snRNP assembly factor in yeast and human, is required for miRNA accumulation. JANUS associates with MC components Dicer-like 1 and SERRATE (SE) and directly binds the stem-loop of pri-miRNAs. In a hypomorphic janus mutant, the activity of DCL1, the numbers of MC, and the interaction of primary miRNA transcript (pri-miRNAs) with MC are reduced. These data suggest that JANUS promotes the assembly and activity of MC through its interaction with MC and/or pri-miRNAs. In addition, JANUS modulates the transcription of some pri-miRNAs as it binds the promoter of pri-miRNAs and promotes Pol II occupancy of at their promoters. Moreover, global splicing defects are detected in janus. Taken together, our study reveals a novel role of a conserved splicing factor in miRNA biogenesis.
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| Overall design |
Comparative mRNA and miRNA expression profiling analysis for janus mutant and Col-0 wildtype.
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| Contributor(s) |
Li M, Yu B, Zhang C |
| Citation missing |
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| Submission date |
Apr 12, 2023 |
| Last update date |
Jun 03, 2023 |
| Contact name |
Chi Zhang |
| E-mail(s) |
czhang5@unl.edu
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| Organization name |
University of Nebraska - Lincoln
|
| Street address |
1901 Vine Street
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| City |
Lincoln |
| State/province |
NE |
| ZIP/Postal code |
68588 |
| Country |
USA |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA954823 |
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