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| Status |
Public on Jul 23, 2024 |
| Title |
An RdDM-independent function of Pol V transcripts in gene regulation and plant defense |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA polymerase V (Pol V) and Pol IV are known to be specialized for RNA-directed DNA methylation (RdDM). Here, we report that Pol V, but not Pol IV, regulates hundreds of genes including JA-responsive genes and confers plant defense to Botrytis cinerea and Spodoptera exigua. About half of the Pol V-regulated genes are associated with Pol V transcripts (PVTs). We thus hypothesized that some PVTs could regulate gene expression in an RdDM-independent manner. To test this hypothesis, we studied three PVTs, PVT-ERF5a/b and PVT-ERF6, as models. PVT-ERF5a/b and PVT-ERF6 are transcribed from the upstream regions of ERF5 and ERF6 and positively regulate their transcription, thereby regulating plant defense. We further show that such regulation involves PVT-dependent H3K4me3 deposition and requires the DRD1-DMS3-RDM1 complex that mediates Pol V recruitment to the target loci. Our findings highlight an unprecedented role for PVTs in regulating gene transcription, apart from serving as scaffold RNAs to direct DNA methylation.
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| |
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| Overall design |
RNA-seq of 2-week-old seedlings of Col-0, nrpd1-3, nrpe1-11
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| Contributor(s) |
Yuan Y, Liu Y, Han L, Li Y, Qi Y |
| Citation missing |
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| |
| Submission date |
Apr 17, 2023 |
| Last update date |
Jul 23, 2024 |
| Contact name |
Yijun Qi |
| E-mail(s) |
qiyijun@mail.tsinghua.edu.cn
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| Organization name |
Tsinghua University
|
| Department |
School of Life Sciences
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| Street address |
NO.1 Qinghuayuan
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| City |
Beiing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA956519 |
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