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| Status |
Public on May 17, 2023 |
| Title |
Non-pathogenic leaf-colonising bacteria elicit pathogen-like responses in a colonisation density-dependent manner |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Leaves are colonised by a complex mix of microbes, termed the leaf microbiota. Even though the leaf microbiota is increasingly recognised as an integral part of plant life and health, our understanding of its interactions with the plant host is still limited. Here, mature, axenically grown Arabidopsis thaliana plants were spray-inoculated with diverse leaf-colonising bacteria. Whole transcriptome sequencing revealed that four days after inoculation, leaf transcriptional changes to colonisation by non-pathogenic and pathogenic bacteria differed in strength but not in the type of response. Inoculation of plants with different densities of the non-pathogenic bacterium Williamsia sp. Leaf354 showed that high bacterial titers caused disease phenotypes and led to severe transcriptional reprogramming with a strong focus on plant defence.
This SuperSeries is composed of the SubSeries listed below.
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| Overall design |
Refer to individual Series
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| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 11, 2023 |
| Last update date |
May 17, 2023 |
| Contact name |
Moritz Miebach |
| E-mail(s) |
miebach.mor@gmail.com
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| Phone |
+64 2041694314
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| Organization name |
University of Canterbury
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| Department |
School of Biological Sciences
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| Street address |
20 Kirkwood Avenue, University of Canterbury Private Bag 4800
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| City |
Christchurch, Canterbury |
| State/province |
Canterbury |
| ZIP/Postal code |
8041 |
| Country |
New Zealand |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (27)
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| GSM7324498 |
aboveground plant parts, mock-treatment (PBS), biol. rep. 1, exp.2 |
| GSM7324499 |
aboveground plant parts, mock-treatment (PBS), biol. rep. 2, exp.2 |
| GSM7324500 |
aboveground plant parts, mock-treatment (PBS), biol. rep. 3, exp.2 |
| GSM7324501 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354 at 10^5 cfu/ml, biol. rep. 1, exp.2 |
| GSM7324502 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354 at 10^5 cfu/ml, biol. rep. 2, exp.2 |
| GSM7324503 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354 at 10^5 cfu/ml, biol. rep. 3, exp.2 |
| GSM7324504 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354 at 10^6 cfu/ml, biol. rep. 1, exp.2 |
| GSM7324505 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354 at 10^6 cfu/ml, biol. rep. 2, exp.2 |
| GSM7324506 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354 at 10^6 cfu/ml, biol. rep. 3, exp.2 |
| GSM7324507 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354 at 10^7 cfu/ml, biol. rep. 1, exp.2 |
| GSM7324508 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354 at 10^7 cfu/ml, biol. rep. 2, exp.2 |
| GSM7324509 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354 at 10^7 cfu/ml, biol. rep. 3, exp.2 |
| GSM7324510 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354 at 10^8 cfu/ml, biol. rep. 1, exp.2 |
| GSM7324511 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354 at 10^8 cfu/ml, biol. rep. 2, exp.2 |
| GSM7324512 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354 at 10^8 cfu/ml, biol. rep. 3, exp.2 |
| GSM7324586 |
aboveground plant parts, mock-treatment (PBS), biol. rep. 1, exp.1 |
| GSM7324587 |
aboveground plant parts, mock-treatment (PBS), biol. rep. 2, exp.1 |
| GSM7324588 |
aboveground plant parts, mock-treatment (PBS), biol. rep. 3, exp.1 |
| GSM7324589 |
aboveground plant parts, infected with Pst, biol. rep. 1, exp.1 |
| GSM7324590 |
aboveground plant parts, infected with Pst, biol. rep. 2, exp.1 |
| GSM7324591 |
aboveground plant parts, infected with Pst, biol. rep. 3, exp.1 |
| GSM7324592 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354, biol. rep. 1, exp.1 |
| GSM7324593 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354, biol. rep. 2, exp.1 |
| GSM7324594 |
aboveground plant parts, inoculated with Williamsia sp. Leaf354, biol. rep. 3, exp.1 |
| GSM7324595 |
aboveground plant parts, inoculated with Microbacterium sp. Leaf347, biol. rep. 1, exp.1 |
| GSM7324596 |
aboveground plant parts, inoculated with Microbacterium sp. Leaf347, biol. rep. 2, exp.1 |
| GSM7324598 |
aboveground plant parts, inoculated with Microbacterium sp. Leaf347, biol. rep. 3, exp.1 |
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| This SuperSeries is composed of the following SubSeries: |
| GSE232248 |
Non-pathogenic leaf-colonising bacteria elicit plant immune responses in a colonisation density-dependent manner |
| GSE232251 |
Non-pathogenic and pathogenic leaf-colonising bacteria elicit qualitatively similar responses |
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| Relations |
| BioProject |
PRJNA971483 |
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