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| Status |
Public on May 15, 2023 |
| Title |
Lymph-Node-Based CD3+ CD20+ Cells Emerge from Membrane Exchange between T Follicular Helper Cells and B Cells and Increase Their Frequency following Simian Immunodeficiency Virus Infection |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by array
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| Summary |
CD4+ T follicular helper (TFH) cells are key targets for human immunodeficiency virus (HIV)/simian immunodeficiency virus (SIV) replication and contribute to the virus reservoir under antiretroviral therapy (ART). Here, we describe a novel CD3+ CD20+ double-positive (DP) lymphocyte subset, resident in secondary lymphoid organs of humans and rhesus macaques (RMs), that appear predominantly after membrane exchange between TFH and B cells. DP lymphocytes are enriched in cells displaying a TFH phenotype (CD4+ PD1hi CXCR5hi), function (interleukin 21 positive [IL-21+]), and gene expression profile. Importantly, expression of CD40L upon brief in vitro mitogen stimulation identifies, by specific gene- expression signatures, DP cells of TFH-cell origin versus those of B-cell origin. Analysis of 56 RMs showed that DP cells (i) significantly increase following SIV infection, (ii) are reduced after 12 months of ART in comparison to pre-ART levels, and (iii) expand to a significantly higher frequency following ART interruption. Quantification of total SIV-gag DNA on sorted DP cells from chronically infected RMs showed that these cells are susceptible to SIV infection. These data reinforce earlier observations that CD20+ T cells are infected and expanded by HIV infection, while suggesting that these cells phenotypically overlap activated CD4+ TFH cells that acquire CD20 expression via trogocytosis and can be targeted as part of therapeutic strategies aimed at HIV remission.
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| Overall design |
LN cells from 9 Rhesus Macaques were FACS-sorted and gene expresson profiles were examined.
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| Web link |
https://doi.org/10.1128/jvi.01760-22
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| Contributor(s) |
Samer S, Chowdhury A, Wiche Salinas TR, Del Rio Estrada PM, Reuter M, Tharp G, Bosinger S, Cervasi B, Auger J, Gill K, Ablanedo-Terrazas Y, Reyes-Teran G, Estes JD, Betts MR, Silvestri G, Paiardini M |
| Citation(s) |
37223960 |
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| Submission date |
May 15, 2023 |
| Last update date |
Aug 14, 2023 |
| Contact name |
Gregory K Tharp |
| E-mail(s) |
gktharp@emory.edu
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| Phone |
404-727-7797
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| Organization name |
Yerkes National Primate Research Center
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| Department |
Developmental and Cognitive Neuroscience
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| Lab |
Genomics Core
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| Street address |
954 Gatewood Dr
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| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30329-4208 |
| Country |
USA |
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| Platforms (1) |
| GPL3535 |
[Rhesus] Affymetrix Rhesus Macaque Genome Array |
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| Samples (50)
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| Relations |
| BioProject |
PRJNA972751 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE232577_RAW.tar |
242.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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