|
| Status |
Public on May 15, 2023 |
| Title |
B5_RCj13B2 |
| Sample type |
RNA |
| |
|
| Source name |
sorted lymphocyte
|
| Organism |
Macaca mulatta |
| Characteristics |
subject_id: RCj13
cell type: sorted lymphocyte
subset: TFH
stimulation: Unstim
|
| Treatment protocol |
brief PMA/ionomycin stimulation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using the RNeasy minikit (Qiagen, Hilden, Germany). The integrity and quantity of the extracted RNA were assessed with an Agilent bioanalyzer (Agilent Technologies, Santa Clara, CA) and NanoDrop 2000 spectrophotometer (Thermo Scientific, Inc., Wilmington, DE).
|
| Label |
biotin
|
| Label protocol |
Five micrograms of the amplified cDNA was fragmented, bio- tinylated, and hybridized to arrays using the NuGEN Ovation Encore biotin module according to kit instruc- tions.
|
| |
|
| Hybridization protocol |
Arrays were washed, stained, and scanned as described in the Affymetrix GeneChip expression analysis technical manual
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
Background adjustment, normalization, and median polish summarization of CEL files were performed with the robust multichip average (RMA) algorithm using the “affy” Bioconductor package
|
| |
|
| Submission date |
May 15, 2023 |
| Last update date |
May 15, 2023 |
| Contact name |
Gregory K Tharp |
| E-mail(s) |
gktharp@emory.edu
|
| Phone |
404-727-7797
|
| Organization name |
Yerkes National Primate Research Center
|
| Department |
Developmental and Cognitive Neuroscience
|
| Lab |
Genomics Core
|
| Street address |
954 Gatewood Dr
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30329-4208 |
| Country |
USA |
| |
|
| Platform ID |
GPL3535 |
| Series (1) |
| GSE232577 |
Lymph-Node-Based CD3+ CD20+ Cells Emerge from Membrane Exchange between T Follicular Helper Cells and B Cells and Increase Their Frequency following Simian Immunodeficiency Virus Infection |
|
