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| Status |
Public on May 25, 2024 |
| Title |
Effect of ARL11 overexpression on gene expression of MDA-MB-231 cells during olaparib treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the function ARL11 in the regulation of PARPi resistance, we established MDA-MB-231 cells overexpressing ARL11. We then performed gene expression profiling analysis using data obtained from RNA-seq of ARL11 overexpression or vector control MDA-MB-231 cells treated with or without Olaparib.
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| Overall design |
5×10^5 ARL11 overexpression or vector control MDA-MB-231 cells were plated on 10 cm dishes and cultured for 24 hours. Cells were then treated with either 40 μM DMSO or Olaparib for 3 days. Cells were lysed for RNA extraction and cDNA library construction and RNA-seq analysis;
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| Contributor(s) |
Zhang T, Zheng H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 25, 2023 |
| Last update date |
May 25, 2024 |
| Contact name |
Hanqiu Zheng |
| E-mail(s) |
hanzheng@tsinghua.edu.cn
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| Organization name |
Tsinghua university
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| Street address |
Haidian District
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| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (4)
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| GSM7427924 |
MDA-MB-231 cells, Vector control, DMSO treatment |
| GSM7427925 |
MDA-MB-231 cells, ARL11 overexpression, DMSO treatment |
| GSM7427926 |
MDA-MB-231 cells, Vector control, Olaparib treatment |
| GSM7427927 |
MDA-MB-231 cells, ARL11 overexpression, Olaparib treatment |
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| Relations |
| BioProject |
PRJNA976247 |
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