|
| Status |
Public on May 25, 2024 |
| Title |
MDA-MB-231 cells, Vector control, Olaparib treatment |
| Sample type |
SRA |
| |
|
| Source name |
MDA-MB-231
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
cell type: human breast cancer cell line
genotype: WT
treatment: 40 μM Olaparib for 3 days
|
| Treatment protocol |
Cells were then treated with either 40 μM DMSO or Olaparib for 3 days.
|
| Growth protocol |
MDA-MB-231 cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was collected using Trizol (Takara, Cat # 9108) according to the manufacturer’s instruction.
Libraries were constructed using NEBNext Ultra II Directional RNA library Prep Kit for Illumina according to the manufacturer’s instruction (NEB, Cat # E7765S).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Vector_Olaparib
|
| Data processing |
The quality of Illumina RNA sequences were controlled by using FastQC
Illumina adapter sequences have been trimmed off
the remaining sequences were subsequently mapped against the GRCh38 human reference using STAR
The output SAM files were converted into sort BAM files by Samtools
The counts for each gene were obtained by using featureCounts
Assembly: GRCh38 human
Supplementary files format and content: CSV files include CPM normalized counts for each Sample
|
| |
|
| Submission date |
May 25, 2023 |
| Last update date |
May 25, 2024 |
| Contact name |
Hanqiu Zheng |
| E-mail(s) |
hanzheng@tsinghua.edu.cn
|
| Organization name |
Tsinghua university
|
| Street address |
Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE233429 |
Effect of ARL11 overexpression on gene expression of MDA-MB-231 cells during olaparib treatment |
|
| Relations |
| BioSample |
SAMN35369910 |
| SRA |
SRX20519681 |
