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| Status |
Public on Jun 01, 2023 |
| Title |
In vivo inhibition of nuclear ACE2 translocation protects against SARS-CoV-2 replication and lung damage through epigenetic imprinting |
| Organisms |
Mesocricetus auratus; unidentified |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In vitro, ACE2 translocates to the nucleus to induce SARS-CoV-2 replication. Here, using digital spatial profiling of lung tissues from SARS-CoV-2-infected golden Syrian hamsters, we show that a specific and selective peptide inhibitor of nuclear ACE2 (NACE2i) inhibits viral replication two days after SARS-CoV-2 infection.
NACE2i also prevents inflammation and macrophage infiltration, and increases NK cell infiltration in bronchioles. NACE2i treatment restores host translation in infected hamster bronchiolar cells.
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| Overall design |
Comparative gene expression profiling analysis of spatial RNA-seq data for the selected regions of interest (ROIs) on the lung tissues of SARS-CoV-2 infected hamster with or without NACE2i treatment .
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| Contributor(s) |
Rao S, Tu WJ, Melino M, Dunn J, McCuaig RD, Bielefeldt-Ohmann H, Tsimbalyuk S, Forwood JK, Ahuja T, Vandermeide J, Tan X, Tran M, Nguyen Q, Zhang L, Nam A, Pan L, Liang Y, Smith C, Lineburg K, Nguyen TH, Sng JD, Tong ZW, Chew KY, Short KR, Le Grand R, Seddiki N |
| Citation(s) |
37369668 |
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| Submission date |
May 29, 2023 |
| Last update date |
Sep 11, 2023 |
| Contact name |
Quan Nguyen |
| E-mail(s) |
quan.nguyen@uq.edu.au
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| Phone |
61452358651
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| Organization name |
Institute for Molecular Bioscience
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| Street address |
22 Burns Street, Indooroopilly
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| City |
Brisbane |
| State/province |
Queensland |
| ZIP/Postal code |
4068 |
| Country |
Australia |
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| Platforms (2) |
| GPL28997 |
Illumina NovaSeq 6000 (Mesocricetus auratus) |
| GPL32371 |
Illumina NovaSeq 6000 (unidentified) |
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| Samples (49)
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| GSM7431805 |
Control, lung bronchiole_H06 3 |
| GSM7431806 |
Control, lung bronchiole_H06 4 |
| GSM7431807 |
Control, lung bronchiole_H06 5 |
| GSM7431808 |
Control, lung bronchiole_H06 6 |
| GSM7431809 |
Control, lung alveoli_H06 1 |
| GSM7431810 |
Control, lung alveoli_H06 2 |
| GSM7431811 |
Control, lung alveoli_H06 3 |
| GSM7431812 |
Control, lung alveoli_H06 4 |
| GSM7431813 |
Control, lung alveoli_H06 5 |
| GSM7431814 |
Control, lung alveoli_H06 6 |
| GSM7431815 |
NACE2i treatment, lung bronchiole_H017 1 |
| GSM7431816 |
NACE2i treatment, lung bronchiole_H017 2 |
| GSM7431817 |
NACE2i treatment, lung bronchiole_H017 3 |
| GSM7431818 |
NACE2i treatment, lung bronchiole_H017 4 |
| GSM7431819 |
NACE2i treatment, lung bronchiole_H017 5 |
| GSM7431820 |
NACE2i treatment, lung bronchiole_H017 6 |
| GSM7431821 |
NACE2i treatment, lung alveoli_H017 1 |
| GSM7431822 |
NACE2i treatment, lung alveoli_H017 2 |
| GSM7431823 |
NACE2i treatment, lung alveoli_H017 3 |
| GSM7431824 |
NACE2i treatment, lung alveoli_H017 4 |
| GSM7431825 |
NACE2i treatment, lung alveoli_H017 5 |
| GSM7431826 |
NACE2i treatment, lung alveoli_H017 6 |
| GSM7431827 |
Control, lung bronchiole_H05 1 |
| GSM7431828 |
Control, lung bronchiole_H05 2 |
| GSM7431829 |
Control, lung bronchiole_H05 3 |
| GSM7431830 |
Control, lung bronchiole_H05 4 |
| GSM7431831 |
Control, lung bronchiole_H05 5 |
| GSM7431832 |
Control, lung bronchiole_H05 6 |
| GSM7431833 |
Control, lung alveoli_H05 1 |
| GSM7431834 |
Control, lung alveoli_H05 2 |
| GSM7431835 |
Control, lung alveoli_H05 3 |
| GSM7431836 |
Control, lung alveoli_H05 4 |
| GSM7431837 |
Control, lung alveoli_H05 5 |
| GSM7431838 |
Control, lung alveoli_H05 6 |
| GSM7431839 |
NACE2i treatment, lung bronchiole_H023 1 |
| GSM7431840 |
NACE2i treatment, lung bronchiole_H023 2 |
| GSM7431841 |
NACE2i treatment, lung bronchiole_H023 3 |
| GSM7431842 |
NACE2i treatment, lung bronchiole_H023 4 |
| GSM7431843 |
NACE2i treatment, lung bronchiole_H023 5 |
| GSM7431844 |
NACE2i treatment, lung bronchiole_H023 6 |
| GSM7431845 |
NACE2i treatment, lung alveoli_H023 1 |
| GSM7431846 |
NACE2i treatment, lung alveoli_H023 2 |
| GSM7431847 |
NACE2i treatment, lung alveoli_H023 3 |
| GSM7431848 |
NACE2i treatment, lung alveoli_H023 4 |
| GSM7431849 |
NACE2i treatment, lung alveoli_H023 5 |
| GSM7431850 |
NACE2i treatment, lung alveoli_H023 6 |
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| Relations |
| BioProject |
PRJNA977210 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE233642_Covid19_Q3normalised.txt.gz |
821.1 Kb |
(ftp)(http) |
TXT |
| GSE233642_RAW.tar |
3.2 Mb |
(http)(custom) |
TAR (of DCC) |
| GSE233642_covid19_metadata.csv.gz |
7.1 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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