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| Status |
Public on Aug 01, 2023 |
| Title |
Responses of Atlantic cod to high water temperature and bacterial infection |
| Organism |
Gadus morhua |
| Experiment type |
Expression profiling by array
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| Summary |
Aquaculture is one of the fastest growing food production sectors in the world and further expansion is expected throughout the 21st century. However, climate change is threatening the development of the sector and action is needed to prepare the industry for the coming challenges. Using downscaled temperature projections based on the Intergovernmental Panel on Climate Change (IPCC) climate projection (Shared Socioeconomic Pathway, SSP2-4.5), we analysed potential future temperatures at a selected Atlantic cod (Gadus morhua L.) farm site in Northern Norway. Results showed that the farming area may experience increased temperatures the next 10–15 years, including more days with temperatures above 17°C. Based on the predicted future conditions, we designed a study with Atlantic cod (Gadus morhua L.) to evaluate effects from high temperature alone and in combination with Fransicella noatunensis infection. Fish were kept at 12°C and 17°C for eight weeks and samples of skin and spleen collected at different timepoints were analysed with transcriptomics, histology, scanning electron microscopy and immunohistochemistry.
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| Overall design |
Atlantic cod, produced at the Center of Marine Aquaculture (Nofima, Norway), were transferred to The Aquaculture Research Station (Tromsø, Norway). Fish were kept for acclimatization for 4 weeks in flow-through tanks at 8 oC, and fed Amber Neptun (Skretting, Norway). Temperature increase was performed in two stages. First the fish were acclimated to 12oC for one week. Second, 120 fish were distributed in 4 tanks, 30 fish per tank, and temperature in two tanks increased to 17 oC. Temperature in the two remaining tanks were kept at 12oC. After acclimatization, fish were moved to the challenge facility at the station where they were kept in 200 L tanks with similar conditions. First day after transfer (T0), three fish from each tank were killed by an overdose of anaesthetics and sampled. At week 8, fish were challenged by cohabitation with Francisella noatunensis (type strain NCIMB 14265).
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| Contributor(s) |
Ytteborg E, Aleksei K |
| Citation |
Ytteborg Elisabeth, Falconer Lynne, Krasnov Aleksei, Johansen Lill-Heidi, Timmerhaus Gerrit, Johansson Gunhild Seljehaug, Afanasyev Sergey, Høst Vibeke, Hjøllo Solfrid Sætre, Hansen Øyvind J, and Lazado Carlo C. Climate change with increasing seawater temperature will challenge the health of farmed Atlantic Cod (Gadus morhua L.). Frontiers in Marine Science 2023, Vol 10. doi:103389/fmars20231232580
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| Submission date |
May 30, 2023 |
| Last update date |
Oct 31, 2023 |
| Contact name |
Aleksei Krasnov |
| E-mail(s) |
aleksei.krasnov@nofima.no
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| Phone |
97602165
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| Organization name |
Nofima AS
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| Street address |
Øreveien, 47B
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| City |
Ås |
| State/province |
Viken |
| ZIP/Postal code |
1523 |
| Country |
Norway |
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| Platforms (1) |
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| Samples (59)
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| GSM7433540 |
Skin, before trial, 8°C, fish 4 |
| GSM7433541 |
Skin, week 1, 12°C, fish 10 |
| GSM7433542 |
Skin, week 1, 12°C, fish 11 |
| GSM7433543 |
Skin, week 1, 12°C, fish 6 |
| GSM7433544 |
Skin, week 1, 12°C, fish 7 |
| GSM7433545 |
Skin, week 1, 12°C, fish 9 |
| GSM7433546 |
Skin, week 1, 17°C, fish 12 |
| GSM7433547 |
Skin, week 1, 17°C, fish 13 |
| GSM7433548 |
Skin, week 1, 17°C, fish 15 |
| GSM7433549 |
Skin, week 1, 17°C, fish 16 |
| GSM7433550 |
Skin, week 1, 17°C, fish 17 |
| GSM7433551 |
Skin, week 8, 12°C, fish 73 |
| GSM7433552 |
Skin, week 8, 12°C, fish 74 |
| GSM7433553 |
Skin, week 8, 12°C, fish 75 |
| GSM7433554 |
Skin, week 8, 12°C, fish 76 |
| GSM7433555 |
Skin, week 8, 17°C, fish 84 |
| GSM7433556 |
Skin, week 8, 17°C, fish 85 |
| GSM7433557 |
Skin, week 8, 17°C, fish 86 |
| GSM7433558 |
Skin, week 8, 17°C, fish 87 |
| GSM7433559 |
Skin, week 8, 12°C, challenged fish 66 |
| GSM7433560 |
Skin, week 8, 12°C, challenged fish 67 |
| GSM7433561 |
Skin, week 8, 12°C, challenged fish 68 |
| GSM7433562 |
Skin, week 8, 12°C, challenged fish 70 |
| GSM7433563 |
Skin, week 8, 17°C, challenged fish 78 |
| GSM7433564 |
Skin, week 8, 17°C, challenged fish 79 |
| GSM7433565 |
Skin, week 8, 17°C, challenged fish 81 |
| GSM7433566 |
Skin, week 8, 17°C, challenged fish 82 |
| GSM7433567 |
Spleen, before trial, 8°C, fish 1 |
| GSM7433568 |
Spleen, before trial, 8°C, fish 2 |
| GSM7433569 |
Spleen, before trial, 8°C, fish 3 |
| GSM7433570 |
Spleen, before trial, 8°C, fish 4 |
| GSM7433571 |
Spleen, week 1, 12°C, fish 10 |
| GSM7433572 |
Spleen, week 1, 12°C, fish 11 |
| GSM7433573 |
Spleen, week 1, 12°C, fish 6 |
| GSM7433574 |
Spleen, week 1, 12°C, fish 7 |
| GSM7433575 |
Spleen, week 1, 12°C, fish 9 |
| GSM7433576 |
Spleen, week 1, 17°C, fish 12 |
| GSM7433577 |
Spleen, week 1, 17°C, fish 13 |
| GSM7433578 |
Spleen, week 1, 17°C, fish 15 |
| GSM7433579 |
Spleen, week 1, 17°C, fish 16 |
| GSM7433580 |
Spleen, week 1, 17°C, fish 17 |
| GSM7433581 |
Spleen, week 8, 12°C, fish 73 |
| GSM7433582 |
Spleen, week 8, 12°C, fish 74 |
| GSM7433583 |
Spleen, week 8, 12°C, fish 75 |
| GSM7433584 |
Spleen, week 8, 12°C, fish 76 |
| GSM7433585 |
Spleen, week 8, 17°C, fish 84 |
| GSM7433586 |
Spleen, week 8, 17°C, fish 85 |
| GSM7433587 |
Spleen, week 8, 17°C, fish 86 |
| GSM7433588 |
Spleen, week 8, 17°C, fish 87 |
| GSM7433589 |
Spleen, week 8, 12°C, challenged fish 66 |
| GSM7433590 |
Spleen, week 8, 12°C, challenged fish 67 |
| GSM7433591 |
Spleen, week 8, 12°C, challenged fish 68 |
| GSM7433592 |
Spleen, week 8, 12°C, challenged fish 70 |
| GSM7433593 |
Spleen, week 8, 17°C, challenged fish 78 |
| GSM7433594 |
Spleen, week 8, 17°C, challenged fish 79 |
| GSM7433595 |
Spleen, week 8, 17°C, challenged fish 82 |
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| Relations |
| BioProject |
PRJNA977676 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE233737_RAW.tar |
118.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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