|
| Status |
Public on Aug 01, 2023 |
| Title |
Spleen, before trial, 8°C, fish 1 |
| Sample type |
RNA |
| |
|
| Source name |
Spleen
|
| Organism |
Gadus morhua |
| Characteristics |
time point: before trial
temperature: 8°C
|
| Treatment protocol |
Treatment included acclimatization to optimum and increased temperature (12°C and 17°C) and challenge with Francisella noatunensis after 8 weeks.
|
| Growth protocol |
Atlantic cod were kept in tanks with sea water
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy® Mini Kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Amplified and labeled with Low Input Quick Amp Labeling Kit (Agilent).
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Arrays were scanned with Agilent G2565BA DNA microarray scanner at a resolution of 5 microns
|
| Description |
fish 1
|
| Data processing |
Images were processed using the standard procedures of the Agilent Feature Extraction (FE) Software version 9.5.1. After removal of flagged spots lowess normalization was performed
|
| |
|
| Submission date |
May 30, 2023 |
| Last update date |
Aug 01, 2023 |
| Contact name |
Aleksei Krasnov |
| E-mail(s) |
aleksei.krasnov@nofima.no
|
| Phone |
97602165
|
| Organization name |
Nofima AS
|
| Street address |
Øreveien, 47B
|
| City |
Ås |
| State/province |
Viken |
| ZIP/Postal code |
1523 |
| Country |
Norway |
| |
|
| Platform ID |
GPL33443 |
| Series (1) |
| GSE233737 |
Responses of Atlantic cod to high water temperature and bacterial infection |
|
