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| Status |
Public on Aug 03, 2010 |
| Title |
Transcription profile of zebrafish hand2 (hanS6 allele) mutant embryos at 19hpf |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
Heart formation requires the fusion of bilateral cardiomyocyte populations as they move toward the embryonic midline. The bHLH transcription factor Hand2 is essential for cardiac fusion; however, the effector genes that execute this function of Hand2 are unknown. Here, we provide the first evidence for a downstream component of the Hand2 pathway that mediates cardiac morphogenesis. Although hand2 is expressed in cardiomyocytes, mosaic analysis demonstrates that hand2 plays a non-autonomous role in regulating cardiomyocyte movement. Gene expression profiles reveal heightened expression of fibronectin 1 (fn1) in hand2 mutant embryos. Reciprocally, overexpression of hand2 leads to decreased Fibronectin levels. Furthermore, reduction of fn1 function enables rescue of cardiac fusion in hand2 mutants: bilateral cardiomyocyte populations merge and exhibit improved tissue architecture, albeit without major changes in apicobasal polarity. Together, our data provide a novel example of a tissue creating a favorable environment for its morphogenesis: the Hand2 pathway establishes an appropriate environment for cardiac fusion through negative modulation of Fn1 levels.
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| Overall design |
Embryos from three independent hand2 mutant (hanS6 allele) heterozyogous crosses were collected. Examination of Tg(myl7:egfp) expression allow sorting of hand2 mutant embryos from their wild-type siblings.
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| Contributor(s) |
Garavito-Aguilar ZV, Yelon D |
| Citation(s) |
20724450 |
| Submission date |
Aug 02, 2010 |
| Last update date |
Jan 25, 2018 |
| Contact name |
Zayra Viviana Garavito-Aguilar |
| E-mail(s) |
zgaravitoaguilar@ucsd.edu
|
| Fax |
(858)534-0555
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| Organization name |
University of California, San Diego
|
| Department |
Division of Biological Sciences
|
| Lab |
Yelon Lab
|
| Street address |
9500 Gilman Drive #0347
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0347 |
| Country |
USA |
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| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA131267 |
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