|
Status |
Public on Aug 03, 2010 |
Title |
zebrafish_han_Mut_Rep1 |
Sample type |
RNA |
|
|
Source name |
hand2 mutant sibling,19hpf
|
Organism |
Danio rerio |
Characteristics |
stage: 19 hpf tissue: Whole embryo
|
Growth protocol |
Embryos were collected, grown in blue water (60 mg/mL Instant Ocean and 0.025% Methylene blue) at 28.5 degrees Celcius and dechorionated prior to RNA extraction
|
Extracted molecule |
total RNA |
Extraction protocol |
3-9mg of total RNA from 10 to 25 embryos per sample were isolated using the RNeasy kit (Qiagen) according to manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
ambion megascript labeling, with biotin labeled dNTP
|
|
|
Hybridization protocol |
Affymetrix standard protocol
|
Scan protocol |
According to manufacturer
|
Data processing |
MAS5, overall intensity was targetted to 500
|
|
|
Submission date |
Aug 02, 2010 |
Last update date |
Aug 02, 2010 |
Contact name |
Zayra Viviana Garavito-Aguilar |
E-mail(s) |
zgaravitoaguilar@ucsd.edu
|
Fax |
(858)534-0555
|
Organization name |
University of California, San Diego
|
Department |
Division of Biological Sciences
|
Lab |
Yelon Lab
|
Street address |
9500 Gilman Drive #0347
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92093-0347 |
Country |
USA |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE23381 |
Transcription profile of zebrafish hand2 (hanS6 allele) mutant embryos at 19hpf |
|