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Series GSE23383 Query DataSets for GSE23383
Status Public on Jul 29, 2011
Title miRNAs in ovarian cancer: A systems approach (miRNA data)
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary MicroRNAs (miRNAs) are short (~22 nucleotides) regulatory RNAs that can modulate gene expression and are aberrantly expressed in many diseases including cancer. We report the results of a systems analysis of miRNA regulation in ovarian cancer. We found that 33 miRNAs are up-regulated and 9 down-regulated in CEPI relative to OSE (p<0.01, ≥2 fold change). Of these, 12 were previously annotated miRNAs (Sanger miRBase) of which 9 are up-regulated and 3 are down-regulated in CEPI relative to OSE. Current models predict that changes in levels of miRNAs will be inversely correlated with changes in the levels of targeted mRNAs due to miRNA regulation. This predicted inverse correlation held for only ~9% of predicted target mRNAs. Computational analyses indicate the unexpected low inverse correlation may be at least partially explained by variation in the number of miRNA binding sites within the 3’ UTRs of targeted mRNAs and by miRNA-mediated changes in levels of transcription factors that can exert overriding trans-regulatory controls on target loci.
Overall design miRNAs were collected from three laser captured microdissected ovarian cancer epithelial (CEPI) samples. The miRNA expression pattern was compared with three healthy ovarian surface epithelia samples as controls using a custom-manufactured Affymetrix GeneChip® array.
Contributor(s) Shahab S, Hill CG, Mittal VK, Mezencev R, Matyunina L, Walker LD, Bowen NJ, Benigno BB, Mcdonald JF
Citation(s) 21811625
Submission date Aug 02, 2010
Last update date Nov 18, 2013
Contact name Shubin Shahab
Organization name Georgia Institute of Technology
Department Biology
Lab McDonald
Street address 310 Ferst Drive
City Atlanta
State/province GA
ZIP/Postal code 30332
Country USA
Platforms (1)
GPL9735 Ambion 14K miRChip V1
Samples (6)
GSM573599 miRNA CEPI 489
GSM573600 miRNA CEPI 551
GSM573601 miRNA CEPI 588
This SubSeries is part of SuperSeries:
GSE23392 miRNAs in ovarian cancer: A systems approach
BioProject PRJNA133315

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23383_RAW.tar 1.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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