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Series GSE234250 Query DataSets for GSE234250
Status Public on Feb 27, 2024
Title Gene expression profile at single level in tumor draining lymph nodes in wild type (DHO001) versus ATG5LEC-KO mice (DHO002)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We used scSEQ analysis to assess differences in the local tumor draining lymph node microenviroment, both on endothelial cells and immune cells
 
Overall design 13 lymph nodes from wild type mice and 9 from ATG5LEC-KO mice were isolated and digested, endothelial cells (viable CD31+ Ter1119- CD45-) and immune cells (viable CD45+ Ter119- CD31-) were sorted and pooled for further sequencing analysis
 
Contributor(s) Houbaert D, Agostinis P, Roels J, Vandamme N
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Submission date Jun 06, 2023
Last update date Feb 28, 2024
Contact name Jana Roels
E-mail(s) jana.roels@vib.be, niels.vandamme@vib.be
Organization name VIB
Department VIB Single cell core
Street address Technologiepark-Zwijnaarde 71
City Zwijnaarde
ZIP/Postal code 9052
Country Belgium
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM7458210 LymphNode ATG5 WT
GSM7458211 LymphNode ATG5 KO
Relations
BioProject PRJNA980620

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE234250_RAW.tar 96.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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