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| Status |
Public on Feb 27, 2024 |
| Title |
Gene expression profile at single level in tumor draining lymph nodes in wild type (DHO001) versus ATG5LEC-KO mice (DHO002) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used scSEQ analysis to assess differences in the local tumor draining lymph node microenviroment, both on endothelial cells and immune cells
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| Overall design |
13 lymph nodes from wild type mice and 9 from ATG5LEC-KO mice were isolated and digested, endothelial cells (viable CD31+ Ter1119- CD45-) and immune cells (viable CD45+ Ter119- CD31-) were sorted and pooled for further sequencing analysis
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/38554280/
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| |
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| Contributor(s) |
Houbaert D, Agostinis P, Roels J, Vandamme N |
| Citation(s) |
38554280 |
| |
| Submission date |
Jun 06, 2023 |
| Last update date |
May 30, 2024 |
| Contact name |
Jana Roels |
| E-mail(s) |
jana.roels@vib.be, niels.vandamme@vib.be
|
| Organization name |
VIB
|
| Department |
VIB Single cell core
|
| Street address |
Technologiepark-Zwijnaarde 71
|
| City |
Zwijnaarde |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA980620 |
