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| Status |
Public on Jun 25, 2023 |
| Title |
Gene expression of subpopulations arising from a nitrogen down-shift with proline as a nitrogen source in S. cerevisiae |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
When S. cerevisiae cells growing exponentially in rich media (YPD) are shifted to nitrogen-limited media with proline (0.8 mM) as a nitrogen source, they differentiate into two subpopulations of different phenotypes including cell size, growth and survival capabilities. The RNA-seq data presented here are for each subpopulation (5 biological replicates per subpopulation) sampled 1 hour after the shift and separated by FACS.
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| Overall design |
We compare 5 biological replicates for each subpopulation. Strain is a prototrophic version of BY4741 containing a subpopulation marker (pRPL28) identified by scRNAseq.
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| Contributor(s) |
Shabestary K, Ledesma-Amaro R |
| Citation missing |
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| Submission date |
Jun 20, 2023 |
| Last update date |
Jun 25, 2023 |
| Contact name |
Kiyan Shabestary |
| E-mail(s) |
k.shabestary@imperial.ac.uk
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| Organization name |
Imperial College London
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| Street address |
Exhibition Road
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| City |
London |
| ZIP/Postal code |
SW7 2AZ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL27812 |
Illumina NovaSeq 6000 (Saccharomyces cerevisiae) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA985601 |
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