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| Status |
Public on Aug 02, 2011 |
| Title |
Genome wide expression analysis of psaA and psbA tobacco mutant plants. |
| Platform organism |
Solanum lycopersicum |
| Sample organism |
Nicotiana tabacum |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptome analysis in tobacco mutant plants using tomato Genechip Genome array
Tobacco (Nicotiana tabacum cv. Petit Havanna) psaA and psbA deletion mutants were constructed through a targeted deletion of 767 and 1152 nucleotides of coding regions, respectively with two gene cassettes: psbAproR:uidA:psbterR and rrnR:aadA:rbcLterR coding for GUS reporter and spectinomycin selectable marker genes, respectively. Standard established procedures were followed for chloroplast transformation to generate the psaA and psbA deletion mutants based on the homologous recombination. Gene expression profiles in psaA and psbA tobacco mutant plants were analyzed using tomato Genechip Genome array to study the global changes in the expression of genome.
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| Overall design |
Total RNA was isolated from psaA and psbA tobacco mutant plants along with the wild type plants. Biotin labeled cRNA was hybridized on tomato GeneChip Genome Array following the Affymetrix protocols. Two independent biological replicates were maintained.
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| Contributor(s) |
Padmalatha KV, Dhandapani G, Kanakachari M, Neha J, Amolkumar SU, Siva RV, Ananda KP |
| Citation(s) |
21327516 |
| |
| Submission date |
Aug 13, 2010 |
| Last update date |
Dec 24, 2015 |
| Contact name |
Padmalatha K.V. |
| E-mail(s) |
kv.padmalatha@gmail.com
|
| Phone |
09953789208
|
| Organization name |
NRCPB
|
| Department |
Plant Biotechnology
|
| Lab |
Bt.lab
|
| Street address |
Pusa campus
|
| City |
New delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110012 |
| Country |
India |
| |
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| Platforms (1) |
| GPL4741 |
[Tomato] Affymetrix Tomato Genome Array |
|
| Samples (6)
|
| GSM579198 |
Tobacco psaA deletion mutant, biological rep 1 |
| GSM579199 |
Tobacco psaA deletion mutant, biological rep 2 |
| GSM579200 |
Tobacco psbA deletion mutant, biological rep 1 |
| GSM579201 |
Tobacco psbA deletion mutant, biological rep 2 |
| GSM579202 |
Tobacco normal plant, biological rep 1 |
| GSM579203 |
Tobacco normal plant, biological rep 2 |
|
| Relations |
| BioProject |
PRJNA129941 |
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