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Series GSE236964 Query DataSets for GSE236964
Status Public on May 24, 2024
Title Heterochromatic 3D genome organization is directed by HP1a and H3K9-dependent and independent mechanisms[CUT&RUN]
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Whether and how histone post-translational modifications and the proteins that bind them drive 3D genome organization remains unanswered. Here, we evaluate the contribution of H3K9-methylated constitutive heterochromatin to 3D genome organization in Drosophila tissues. We find that the predominant organizational feature of wildtype tissues is segregation of euchromatic chromosome arms from heterochromatic pericentromeres. Reciprocal perturbation of HP1a•H3K9me binding, using a point mutation in the HP1a chromodomain or replacement of the replication-dependent histone H3 with H3K9R mutant histones, revealed that HP1a binding to methylated H3K9 in constitutive heterochromatin is required to limit contact frequency between pericentromeres and chromosome arms and regulate the distance between arm and pericentromeric regions. Surprisingly, self-association of pericentromeric regions is largely preserved despite loss of H3K9 methylation and HP1a occupancy. Thus, the HP1a•H3K9 interaction contributes to, but does not solely drive, segregation of euchromatin and heterochromatin inside the nucleus.
Overall design Comparative analysis of CUT&RUN data collected from HP1a chromdomain mutant wing imaginal discs and their controls.
Contributor(s) Stutzman A, Hill C, Armstrong R, Gohil R, Duronio R, Dowen J, McKay D
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Submission date Jul 10, 2023
Last update date May 24, 2024
Contact name Daniel McKay
Organization name University of North Carolina at Chapel Hill
Street address 250 Bell Tower Drive
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
Platforms (1)
GPL21306 Illumina HiSeq 4000 (Drosophila melanogaster)
Samples (51)
GSM7589758 Wing disc, HP1a-control, H3K9me3, rep1
GSM7589759 Wing disc, HP1a-control, H3K9me3, rep2
GSM7589760 Wing disc, HP1a-control, H3K9me3, rep3
This SubSeries is part of SuperSeries:
GSE234277 Heterochromatic 3D genome organization is directed by HP1a and H3K9-dependent and independent mechanisms.
BioProject PRJNA993194

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource 61.7 Mb (ftp)(http) BW 4.3 Mb (ftp)(http) BW
GSE236964_H3K9me3-peaks.bed.gz 43.3 Kb (ftp)(http) BED
GSE236964_HP1-peaks.bed.gz 33.4 Kb (ftp)(http) BED 116.9 Mb (ftp)(http) BW 66.2 Mb (ftp)(http) BW 94.6 Mb (ftp)(http) BW 56.4 Mb (ftp)(http) BW 709.9 Kb (ftp)(http) BW 65.2 Mb (ftp)(http) BW 25.3 Mb (ftp)(http) BW 542.8 Kb (ftp)(http) BW 108.1 Mb (ftp)(http) BW 29.0 Mb (ftp)(http) BW 93.8 Mb (ftp)(http) BW
GSE236964_yw-gfp-blacklist.bed.gz 13.8 Kb (ftp)(http) BED 94.2 Mb (ftp)(http) BW
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Processed data are available on Series record

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