Expression profiling by high throughput sequencing
Summary
scRNAseq of chicken basilar papilla at P7 and P11 as controls, and 12, 16, 20, 24, 30, 38, 48, and 96 hours post sisomicin administration. Cells were isolated using FACS and underwent Smartseq-2 protocol for single cell RNA seq with paired-end sequencing. Processed data file contains SingleCellExperiment object with all metadata. The dataset has undergone quality control using scater package in R, normalized using SCnorm, and normalized counts were log2 transformed (found in RDS file here). Only genes expressed in at least 3 cells are included for a total of 16,302 genes. A total of 2,625 high quality cells are in the normalized dataset. Raw sequence data for all 3,831 sequenced cells pre-quality control is also included.
Overall design
Postnatal day 7 (P7) chicken inner ears were exposed to sisomicin with basilar papilla harvested at indicated timepoints post exposure. Cells underwent FACS sorting prior to library prep and single cell RNA sequencing using the Smartseq2 protocol.
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