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Series GSE242357 Query DataSets for GSE242357
Status Public on Sep 30, 2023
Title MoTrPAC - Endurance Exercise Training Study In 6 Months Old Rats [ATAC-seq]
Organism Rattus norvegicus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The goal of the endurance exercise training study in young adult rats was to perform exercise training studies in young adult (6-month-old) F344 rats, and from these rats collect multiple tissues in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia. For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max and training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
 
Overall design Regular exercise promotes whole-body health and prevents disease, yet the underlying molecular mechanisms throughout a whole organism are incompletely understood. Here, the Molecular Transducers of Physical Activity Consortium (MoTrPAC) profiled the temporal transcriptome, proteome, metabolome, lipidome, phosphoproteome, acetylproteome, ubiquitylproteome, epigenome, and immunome in whole blood, plasma, and 18 solid tissues in Rattus norvegicus over 8 weeks of endurance exercise training. The resulting data compendium encompasses 9466 assays across 19 tissues, 25 molecular platforms, and 4 training time points in young adult male and female rats.

Contributor: Molecular Transducers of Physical Activity Consortium (MoTrPAC)
 
Citation(s) 38697122, 38693412, 38693320
BioProject PRJNA908279
Submission date Sep 05, 2023
Last update date Sep 09, 2024
Contact name Euan Ashley
Organization name Stanford University
Street address 870 Quarry Road
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL25947 Illumina NovaSeq 6000 (Rattus norvegicus)
Samples (416)
GSM7761099 atac-seq_hippoc_male_reference_1_vial_80034885225
GSM7761100 atac-seq_hippoc_female_reference_1_vial_80035995233
GSM7761101 atac-seq_skm-gn_male_reference_2_vial_80000885530
This SubSeries is part of SuperSeries:
GSE242358 MoTrPAC: Endurance Exercise Training Study In 6 Months Old Rats

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE242357_motrpac_pass1b-06_epigen-atac-seq_qa-qc-metrics.csv.gz 102.7 Kb (ftp)(http) CSV
GSE242357_motrpac_pass1b-06_t52-hippocampus_epigen-atac-seq_counts.txt.gz 57.1 Mb (ftp)(http) TXT
GSE242357_motrpac_pass1b-06_t55-gastrocnemius_epigen-atac-seq_counts.txt.gz 59.2 Mb (ftp)(http) TXT
GSE242357_motrpac_pass1b-06_t58-heart_epigen-atac-seq_counts.txt.gz 54.9 Mb (ftp)(http) TXT
GSE242357_motrpac_pass1b-06_t59-kidney_epigen-atac-seq_counts.txt.gz 59.7 Mb (ftp)(http) TXT
GSE242357_motrpac_pass1b-06_t66-lung_epigen-atac-seq_counts.txt.gz 55.9 Mb (ftp)(http) TXT
GSE242357_motrpac_pass1b-06_t68-liver_epigen-atac-seq_counts.txt.gz 54.2 Mb (ftp)(http) TXT
GSE242357_motrpac_pass1b-06_t69-brown-adipose_epigen-atac-seq_counts.txt.gz 57.9 Mb (ftp)(http) TXT
GSE242357_motrpac_pass1b-06_t70-white-adipose_epigen-atac-seq_counts.txt.gz 58.5 Mb (ftp)(http) TXT
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