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| Status |
Public on Sep 13, 2023 |
| Title |
Trunk neural crest gene MOXD1 affects embryonic development |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Ectoderm-derived neural crest is a transient structure arising during early embryogenesis in vertebrates. Neural crest consists of four derivatives based on their anterior- to posterior location along the body axis; cranial, vagal, trunk and sacral, respectively. We recently showed that trunk neural crest-specific gene MOXD1 functions as a tumor suppressor in trunk neural crest-derived childhood cancer form neuroblastoma and is essential for proper development of healthy adrenal glands. However, the role of MOXD1 during early embryogenesis is not known. Here, we conditionally knocked out MOXD1 in trunk neural crest cells before they become lineage-committed, using a CRISPR/Cas9 approach in chick embryos. Assessment of embryo growth showed that knockout of MOXD1 delayed development with knockout embryos being smaller. RNA sequencing of trunk-derived neural crest cells from control and knockout embryos showed enrichment of genes connected to gland development, copper ion metabolism and neuroblastoma progression. In conclusion, MOXD1 is important during early and prolonged embryonic development with effects on gland formation, possibly mediated via its role in copper metabolism.
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| Overall design |
We conditionally knocked out MOXD1 in trunk neural crest cells before they become lineage-committed, using a CRISPR/Cas9 approach in chick embryos. We included nice control embryos and twelve MOXD1 knock-out embryos.
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| Contributor(s) |
Fredlund E, Andersson S, Ferreira M, Mohlin S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Sep 08, 2023 |
| Last update date |
Sep 13, 2023 |
| Contact name |
Stina Andersson |
| E-mail(s) |
stina.andersson@med.lu.se
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| Organization name |
Lund University
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| Street address |
Sölvegatan 19
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| City |
Lund |
| ZIP/Postal code |
22384 |
| Country |
Sweden |
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| Platforms (1) |
| GPL26853 |
Illumina NovaSeq 6000 (Gallus gallus) |
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| Samples (21)
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| GSM7767296 |
Neural tube, CTRL, replicate 4 |
| GSM7767297 |
Neural tube, CTRL, replicate 5 |
| GSM7767298 |
Neural tube, CTRL, replicate 6 |
| GSM7767299 |
Neural tube, CTRL, replicate 7 |
| GSM7767300 |
Neural tube, CTRL, replicate 8 |
| GSM7767301 |
Neural tube, CTRL, replicate 9 |
| GSM7767302 |
Neural tube MOXD1 KO, replicate 1 |
| GSM7767303 |
Neural tube MOXD1 KO, replicate 2 |
| GSM7767304 |
Neural tube MOXD1 KO, replicate 3 |
| GSM7767305 |
Neural tube MOXD1 KO, replicate 4 |
| GSM7767306 |
Neural tube MOXD1 KO, replicate 5 |
| GSM7767307 |
Neural tube MOXD1 KO, replicate 6 |
| GSM7767308 |
Neural tube MOXD1 KO, replicate 7 |
| GSM7767309 |
Neural tube MOXD1 KO, replicate 8 |
| GSM7767310 |
Neural tube MOXD1 KO, replicate 9 |
| GSM7767311 |
Neural tube MOXD1 KO, replicate 10 |
| GSM7767312 |
Neural tube MOXD1 KO, replicate 11 |
| GSM7767313 |
Neural tube MOXD1 KO, replicate 12 |
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| Relations |
| BioProject |
PRJNA1014377 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE242700_2020_75_R2_gene_id.featureCounts.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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