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| Status |
Public on Sep 25, 2010 |
| Title |
DamID for Swi6, Rdp1 and Dcr1 in Schizosaccharomyces pombe |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
In the fission yeast Schizosaccharomyces pombe, the RNA interference (RNAi) pathway is required to generate small interfering RNAs (siRNAs) that mediate heterochromatic silencing of centromeric repeats. Here we demonstrate that RNAi also functions to repress genomic elements other than constitutive heterochromatin. Using DamID (DNA adenine methyltransferase identification) we show that Dcr1 and Rdp1 physically associate with some euchromatic genes, non-coding RNA (ncRNA) genes, and retrotransposon long terminal repeats (LTRs), and that this association is independent of the Clr4 histone methyltransferase. Physical association of RNAi with chromatin is sufficient to trigger a silencing response but not to assemble heterochromatin. The mode of silencing at the newly identified RNAi targets is consistent with a co-transcriptional gene silencing model as proposed earlier and functions with trace amounts of siRNAs. We anticipate that similar mechanisms could also be operational in other eukaryotes.
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| Overall design |
Methylation levels in strains expressing Dam-Swi6, Dam-Rdp1 and Dam-Dcr1 were compared to those in a Dam only expressing strain. Three biological replicates for each experiment were performed.
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| Contributor(s) |
Woolcock KJ, Gaidatzis D, Bühler M |
| Citation(s) |
21151114 |
| |
| Submission date |
Sep 24, 2010 |
| Last update date |
May 27, 2014 |
| Contact name |
Katrina Woolcock |
| Organization name |
Friedrich Miescher Institute for Biomedical Research
|
| Lab |
Bühler
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL7715 |
[Sp20b_M] Affymetrix S. pombe Tiling 1.0FR Array |
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| Samples (29)
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| Relations |
| BioProject |
PRJNA132819 |
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