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| Status |
Public on Apr 02, 2024 |
| Title |
Assessment of myogenic maturation for human iPSC-derived iPAX7 myogenic progenitors in vitro and in vivo |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here, we transplanted human iPAX7 myogenic progenitors into skeletal muscles of non-dystrophic and dystrophic mice and compared the transcriptional landscape of human donor-derived myofibers with respective in vitro-differentiated iPAX7 myotubes. Pairing bulk RNA sequencing with computational deconvolution of human reads, we were able to pinpoint key myogenic changes that occur during the in vitro-to-in vivo transition, confirm developmental maturity, and consequently further suggest the utility of cell-based therapies.
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| Overall design |
We performed gene expression profiling for data obtained through bulk RNA-sequencing of 14 uniques samples. Samples included non-dystrophic or dystrophic skeletal muscle injected with or without iPAX7 myogenic progenitors, as well as terminally differentiated myotubes in vitro.
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| Contributor(s) |
Crist SB, Magli A, Perlingeiro RR |
| Citation(s) |
38575647 |
| Submission date |
Oct 03, 2023 |
| Last update date |
Apr 17, 2024 |
| Contact name |
Sarah Crist |
| E-mail(s) |
crist156@umn.edu
|
| Organization name |
University of Minnesota
|
| Street address |
2231 6th St SE
|
| City |
Minneapolis |
| State/province |
Minnesota |
| ZIP/Postal code |
55455 |
| Country |
USA |
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| Platforms (3) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL25526 |
Illumina NovaSeq 6000 (Homo sapiens; Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA1023472 |
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