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| Status |
Public on Dec 11, 2023 |
| Title |
Cebp1 and Cebpβ transcriptional axis controls eosinophilopoiesis in zebrafish [bulkRNA-Seq] |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Eosinophils are well known to regulate host protection from parasites and have been reported to paticipate many other physiologic and pathologic processes. Understanding the role of eosinophils in these processes requires a better understanding of eosinophilopoiesis. Using a zebrafish model, we have identified an eosinophil lineage-specific marker, eslec. Using this marker we have established a Tg(eslec:eGFP) reporter line, which specifically labels zebrafish eosinophil lineage cells from early life through adulthood. Spatial-temporal analysis of eslec+ cells demonstrated organ distribution at the larval stage. By single-cell RNA-Seq of eslec+ cells, tissue-distributed eosinophils were found to have similar differentiation paths but different tissue-specific expression profiles. Genetic analysis demonstrated a Cebp1 and Cebpβ transcriptional axis that regulated eosinophilopoiesis, in which Cebp1 directly targeted cebpb to inhibit eosinophil differentiationthe commitment, differentiation, and maturation of the eosinophil lineage. In summary, this study characterized eosinophil development in multiple dimensions including spatial-temporal patterns, expression profiles, and genetic regulators. The results provide for a better understanding of eosinophilopoiesis.
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| Overall design |
For the RNA-Seq analysis of eosinophils from different genotypes, eslec:eGFP+ eosinophils were sorted from whole larvae (7 dpf) and lysed. The lysates were directly applied to reverse transcription and amplification. Enriched cDNA were then fragmented, adpator tagged, and purified for further sequencing.
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| Contributor(s) |
Li G, Zhang Y |
| Citation(s) |
38280871 |
| Submission date |
Oct 23, 2023 |
| Last update date |
Mar 11, 2024 |
| Contact name |
Gaofei Li |
| E-mail(s) |
15013156757@126.com
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| Organization name |
South China University of Technology
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| Street address |
Guangzhou Higher Education Mega Centre, Panyu District
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| City |
Guangzhou |
| ZIP/Postal code |
510006 |
| Country |
China |
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| Platforms (1) |
| GPL24995 |
Illumina NovaSeq 6000 (Danio rerio) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE198314 |
Cebp1 and Cebpβ transcriptional axis controls eosinophilopoiesis in zebrafish |
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| Relations |
| BioProject |
PRJNA1031141 |
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