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Series GSE248678 Query DataSets for GSE248678
Status Public on Mar 04, 2024
Title Ribo– RNA-seq of psoriasis mouse model
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Efforts to advance RNA aptamers as a novel therapeutic modality have been limited by their susceptibilty to degradation and immunogenicity. In a previous study, we demonstrated synthesized double-stranded circular RNAs (ds-cRNAs) with minimal immunogenicity targeted to dsRNA-activated Protein Kinase R (PKR). Here, we test the therapeutic potential of ds-cRNAs in a mouse model of imiquimod-induced psoriasis. We find that genetic supplementation of ds-cRNAs leads to inhibition of PKR, resulting in alleviation of downstream interferon alpha (IFNα)/dsRNA signals and attenuation of psoriasis phenotypes. Delivery of ds-cRNAs by lipid nanoparticles to the spleen attenuates PKR activity in examined splenocytes, resulting in reduced epidermal thickness. These findings suggest that ds-cRNAs represent a promising approach to mitigate excessive PKR activation for therapeutic purposes.
Overall design To evaluate the circRNA-PKR axis in psoriasis , we established a psoriasis mouse (C57BL/6) model via IMQ (imiquimod) treatment at day (D) 0, 1, 2, 3, 5. Then we conducted total RNA sequencing of mice spleens at day 0,1,2,5 following IMQ treatment. Of note, each RNA sequencing sample comprised total RNA extracted from two spleens of two mice. Each examined time point has two repeats, and each repeat includes 2 biologically independent mice.
Web link
Contributor(s) Guo S, Liu C, Xu Y, Nan F, Yang L, Chen L
Citation(s) 38653797
Submission date Nov 27, 2023
Last update date Jun 03, 2024
Contact name Li Yang
Organization name Fudan University
Department Institutes of Biological Sciences
Street address 131 Dong-An Road
City Shanghai
ZIP/Postal code 200032
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (16)
GSM7918642 WT mouse with 0 day IMQ treatment ,repeat1
GSM7918643 WT mouse with 0 day IMQ treatment ,repeat2
GSM7918644 WT mouse with 1 day IMQ treatment ,repeat1
This SubSeries is part of SuperSeries:
GSE248680 Therapeutic application of circular RNA aptamers in a mouse model of psoriasis
BioProject PRJNA1045580

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Supplementary file Size Download File type/resource
GSE248678_RAW.tar 67.3 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA

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