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| Status |
Public on Dec 05, 2023 |
| Title |
Genome-wide mapping of MEF2A binding sites by CUT&Tag-seq in chicken primary myoblasts |
| Organism |
Gallus gallus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In this study, CUT&Tag-seq technology was employed to investigate MEF2A binding sites across the entire genome of chicken primary myoblasts. CUT&Tag was performed using CUT&Tag Assay Kit for Illumina Pro (TD904-1) from Vazyme. Antibody targeting MEF2A as well as IgG were used.The final DNA library on a HiSeq PE150 platform was subjected for the analyses. This study provides a wide landscape of MEF2A target genes from chicken primary myoblasts, which supports the active role of MEF2A in avian muscle development.
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| Overall design |
Cleavage under targets and tagmentation (CUT&Tag) for MEF2A in chicken primary myoblasts (CPM)
**Processed data were removed because the submitter stated that the analysis method was wrong**
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| Contributor(s) |
Wang X, Zhang J, Su J, Wang Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 30, 2023 |
| Last update date |
Jul 05, 2024 |
| Contact name |
Xinglong Wang |
| E-mail(s) |
SCU_WXL@outlook.com
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| Phone |
18282722773
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| Organization name |
sichuan university
|
| Department |
College of Life Sciences
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| Lab |
Key laboratory of Bio-resources and Eco-environment of Ministry of Education
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| Street address |
Wangjiang
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| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610065 |
| Country |
China |
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| Platforms (1) |
| GPL26853 |
Illumina NovaSeq 6000 (Gallus gallus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA1047158 |
