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| Status |
Public on Dec 06, 2023 |
| Title |
The expression data from mandibular arch and tongue primordia in mouse embryos. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The mouse hypoglossal nerve originates from the occipital motor nuclei and projects a long distance, reaching the vicinity of the tongue primordia, the lateral lingual swelling, at E11.5. However, the details of how the hypoglossal nerve correctly projects to the primordia are not well understood.
To investigate the molecular basis of hypoglossal nerve elongation, we employed cDNA microarray technology and screened for candidate genes involved in the axon guidance event.
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| Overall design |
Tissues were dissected and pooled together in a single tube for microarray analysis. The following were collected into single tubes: E9.5 median mandibular arches (E9.5-MMA; 12 embryos/tube), E11.5 lateral lingual swellings (E11.5-LLS; 4 embryos/tube), E11.5 lateral mandibular arches (E11.5-LMA; 4 embryos/tube) and E14.5 median tongue (E14.5-T; 4 embryos/tube). Each of these regions were regarded as an independent tissue unit. We applied the ROKU method, an algorithm that can detect tissue-specific genes by comparing expression patters across different time periods and tissue regions.
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| Contributor(s) |
HANI T, FUJITA K, KUDO T, TAYA Y, SATO K, SOENO Y |
| Citation(s) |
38463205 |
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| Submission date |
Dec 06, 2023 |
| Last update date |
Mar 11, 2024 |
| Contact name |
Taisuke Hani |
| E-mail(s) |
t-hani2120004@tky.ndu.ac.jp
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| Organization name |
The Nippon Dental University, School of Life Dentistry at Tokyo
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| Department |
Pathology
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| Street address |
1-9-20
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| City |
Fujimi, Chiyoda-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
155-0032 |
| Country |
Japan |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1049379 |
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