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| Status |
Public on Feb 07, 2024 |
| Title |
A tumor endothelial cell-specific microRNA replacement therapy for hepatocellular carcinoma |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Current approved anti-angiogenic drugs (AAD) for hepatocellular carcinoma (HCC) inhibit tumor angiogenesis, but affect the hepatic vasculature resulting in adverse effects. Tumor endothelial cells (TECs) differ from normal endothelial cells. In this study, we aimed to detect TEC-specific miRNAs and develop an anti-angiogenic treatment specific for TECs. We established HCC orthotopic mouse models. TEC-specific miRNAs were detected using a microRNA array. Finally, we evaluated the therapeutic effects of the TEC-specific miRNA agonist cocktail. In total, 260 TEC-specific genes were detected. Among the top ten downregulated TEC-specific miRNAs, miR-139-3p and 214-3p were important for the TEC phenotype. The TEC-specific microRNA agonist cocktail showed significant anti-tumor effects by inhibiting tumor angiogenesis without affecting hepatic vasculatures in HCC orthotopic mouse models. Moreover, it significantly downregulated tip-cell sprouting-related genes. We identified two downregulated TEC-specific miRNAs; microRNA replacement therapy, which targets the downregulated TEC-specific miRNAs, is an effective and promising treatment for HCC
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| Overall design |
Gene expression of Tumor endothelial cell isolated from mouse orthotopic hepatoma
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| Contributor(s) |
Iwamoto H, Kawaguchi T |
| Citation(s) |
38303694 |
| Submission date |
Dec 13, 2023 |
| Last update date |
Feb 08, 2024 |
| Contact name |
Hideki Iwamoto |
| Organization name |
Kurume University School of Medicine
|
| Department |
Division of Gastroenterology, Department of Medicine
|
| Street address |
67 Asahimachi
|
| City |
Kurume |
| State/province |
Fukuoka |
| ZIP/Postal code |
830-0011 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA1052111 |
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