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Status |
Public on Jan 26, 2011 |
Title |
Genome-wide expression profiling of Fusarium graminearum in response to azole fungicide treatment |
Organism |
Fusarium graminearum |
Experiment type |
Expression profiling by array
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Summary |
In this study, a newly developed 8x15K Fusarium graminearum Agilent microarray was applied to analyze transcriptional responses to azole fungicide treatment. Comparative statistical analysis of expression profiles from treated versus untreated fungal liquid cultures uncovered 1058 genes that were significantly differentially expressed. Among the 596 genes with significantly increased transcript levels, analyses using GeneOntology and FunCat annotations detected the ergosterol-biosynthesis pathway genes as the most significantly responding category, providing statistical confirmation of the mode-of-action of azole fungicides. Transcriptional profiling of 54 genes, which encode ABC transporters in F. graminearum, suggested that several of these might be involved in reducing intracellular fungicide concentration. In addition, several genes encoding transcription factors with similarity to yeast genes known to co-ordinate fungicide responses exhibited significant differential expression.
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Overall design |
Hybridization was performed with cRNA samples either derived from three independent untreated or three independent azole-treated liquid cultures (12 h at 5 mg/l tebuconazole).
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Contributor(s) |
Becher R, Weihmann F, Deising HB, Wirsel SG |
Citation(s) |
21255412 |
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Submission date |
Nov 03, 2010 |
Last update date |
Mar 22, 2012 |
Contact name |
Rayko Becher |
E-mail(s) |
rayko.becher@landw.uni-halle.de
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Organization name |
Martin-Luther-University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences
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Department |
Department of Phytopathology and Plant Protection
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Street address |
Betty-Heimann-Str. 3
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City |
Halle/Saale |
ZIP/Postal code |
06120 |
Country |
Germany |
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Platforms (1) |
GPL11158 |
021301-MLU Fusarium graminearum 8x15K v1.0 |
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Samples (6)
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Relations |
BioProject |
PRJNA134509 |