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Series GSE25114 Query DataSets for GSE25114
Status Public on Jan 26, 2011
Title Genome-wide expression profiling of Fusarium graminearum in response to azole fungicide treatment
Organism Fusarium graminearum
Experiment type Expression profiling by array
Summary In this study, a newly developed 8x15K Fusarium graminearum Agilent microarray was applied to analyze transcriptional responses to azole fungicide treatment. Comparative statistical analysis of expression profiles from treated versus untreated fungal liquid cultures uncovered 1058 genes that were significantly differentially expressed. Among the 596 genes with significantly increased transcript levels, analyses using GeneOntology and FunCat annotations detected the ergosterol-biosynthesis pathway genes as the most significantly responding category, providing statistical confirmation of the mode-of-action of azole fungicides. Transcriptional profiling of 54 genes, which encode ABC transporters in F. graminearum, suggested that several of these might be involved in reducing intracellular fungicide concentration. In addition, several genes encoding transcription factors with similarity to yeast genes known to co-ordinate fungicide responses exhibited significant differential expression.
Overall design Hybridization was performed with cRNA samples either derived from three independent untreated or three independent azole-treated liquid cultures (12 h at 5 mg/l tebuconazole).
Contributor(s) Becher R, Weihmann F, Deising HB, Wirsel SG
Citation(s) 21255412
Submission date Nov 03, 2010
Last update date Mar 22, 2012
Contact name Rayko Becher
Organization name Martin-Luther-University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences
Department Department of Phytopathology and Plant Protection
Street address Betty-Heimann-Str. 3
City Halle/Saale
ZIP/Postal code 06120
Country Germany
Platforms (1)
GPL11158 021301-MLU Fusarium graminearum 8x15K v1.0
Samples (6)
GSM617106 PH1_untreated_rep1
GSM617107 PH1_untreated_rep2
GSM617108 PH1_untreated_rep3
BioProject PRJNA134509

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25114_RAW.tar 13.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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