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| Status |
Public on Nov 23, 2010 |
| Title |
Subcellular expression profiling of the growth cones of retinal ganglion cells (RGC) |
| Organisms |
Xenopus laevis; Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Cue-directed axon guidance depends partly on local translation in growth cones. Many mRNA transcripts are known to reside in developing axons yet little is known about their subcellular distribution or, specifically, which transcripts are in growth cones.
Laser capture microdissection (LCM) was used to isolate the growth cones of retinal ganglion cell (RGC) axons of two vertebrate species, mouse and Xenopus, coupled with unbiased genome-wide microarray profiling.
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| Overall design |
Localized mRNA from the isolated growth cones of Xenopus laevis and Mus musculus retinal ganglion cells were subjected to microarray analysis
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| Contributor(s) |
Zivraj KH, Tung YC, Piper M, Gumy L, Fawcett JW, Yeo GS, Holt CE |
| Citation(s) |
21084603 |
| |
| Submission date |
Nov 05, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Brian Yee Hong Lam |
| E-mail(s) |
yhbl2@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Metabolic Research Laboratories
|
| Street address |
Box 289 Addenbookes Hospital, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QQ |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
| GPL1318 |
[Xenopus_laevis] Affymetrix Xenopus laevis Genome Array |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA134423 |
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