|
| Status |
Public on Nov 23, 2010 |
| Title |
xenopus_axons_2 |
| Sample type |
RNA |
| |
|
| Source name |
Axons of retinal ganglion cells
|
| Organism |
Xenopus laevis |
| Characteristics |
cell type: retinal ganglion
tissue: axons of retinal ganglion cells
|
| Growth protocol |
Whole eye primordia from stage 24 and 32 Xenopus embryos were cultured on polyethylene terephthalate membrane slides for 17h and 20 h respectively. Mouse retinal explants were obtained from E15 embryos and were cultured for 24 h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted from laser capture microdissected samples using RNAqueous Microkit (Ambion) and were amplified using the two-cycle TargetAmp kit (Epicentre)
|
| Label |
biotin
|
| Label protocol |
aRNA were biotin-labelled using GeneChip IVT labeling kit (Affymetrix)
|
| |
|
| Hybridization protocol |
15ug of biotin-labelled RNA were hybridized onto Xenopus Laevis or Mouse 430 2.0 arrays for 16 h at 45C. Followed by washing and staining using Affymetrix Fluidics Station FS450 according to manufacturing instructions
|
| Scan protocol |
The arrays were scanned by Affymetrix GeneChip Scanner 3000 7G using AGCC Software 2.0
|
| Description |
localized mRNA from the axons of retinal ganglion cells
|
| Data processing |
The arrays were normalized by robust multiarray average (RMA) using Affymetrix Expression Console software.
|
| |
|
| Submission date |
Nov 05, 2010 |
| Last update date |
Nov 23, 2010 |
| Contact name |
Brian Yee Hong Lam |
| E-mail(s) |
yhbl2@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Metabolic Research Laboratories
|
| Street address |
Box 289 Addenbookes Hospital, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1318 |
| Series (1) |
| GSE25166 |
Subcellular expression profiling of the growth cones of retinal ganglion cells (RGC) |
|
