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| Status |
Public on Jan 19, 2024 |
| Title |
Phytochrome C and Low Temperature Promote the Expression and Red Light Signaling of Phytochrome D |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Light is an ever-changing environmental parameter affecting almost every aspects of plant growth and development. It is perceived by photoreceptors, among them phytochromes (PHY) are responsible for monitoring the red and far-red part of the spectrum. Arabidopsis thaliana possesses five phytochromes genes, named through phyA-phyE. Whereas the function of phyA and phyB – that mediate most of the phytochrome responses – is extensively studied, our knowledge on other phytochromes are still rudimentary. To analyze phyD function we expressed it at high levels in different phytochrome deficient genetic backgrounds. We found that overexpressed phyD governs effective light signaling at low temperatures but only in cooperation with functional phyC. Under these conditions, opposite to phyB, phyD accumulates to high levels and this pool is stable under light illumination. Furthermore, the detectable photoconvertible phyD amount is proportional with the available protein amount indicating that the phyD pool contains fully functional photoreceptors. The thermal reversion of phyD is very fast suggesting that the thermosensing of phyD is based on its protein amount and not on its Pfr conformer stability, which was described for phyB. We also found that phyD and phyB associate to identical genomic locations and mediate similar gene expression changes, however the efficiency of phyD is lower. Taken together our data suggest that under certain conditions synergistic interaction of phyD and phyC substitutes phyB function thus increases the ability of plants to respond more flexible to environmental changes.
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| Overall design |
Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) with anti-GFP antibody that recognise PHYD-YFP and PHYB-GFP transgenic protein.
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| Contributor(s) |
Grézal G, Péter C, Hajdu A, Kozma-Bognár L, Silhavy D, Nagy F, Ádám É, Viczián A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 15, 2024 |
| Last update date |
Jan 19, 2024 |
| Contact name |
Gabor Grezal |
| E-mail(s) |
grezal.gabor@gmail.com
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| Phone |
0036303063101
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| Organization name |
Biological Research Centre
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| Department |
Institute of Biochemistry
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| Lab |
Balázs Papp Laboratory
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| Street address |
Temesvári krt. 62.
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| City |
Szeged |
| State/province |
Csongrád |
| ZIP/Postal code |
6701 |
| Country |
Hungary |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (9)
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| GSM8016845 |
PHYD-YFP/abcde, 5 minutes, rep 2 |
| GSM8016846 |
PHYD-YFP/abcde, 5 minutes, rep 3 |
| GSM8016847 |
PHYD-YFP/ab-C-de, 5 minutes, rep 2 |
| GSM8016848 |
PHYD-YFP/ab-C-de, 5 minutes, rep 3 |
| GSM8016849 |
PHYB-GFP/AbCDE, 5 minutes, rep 2 |
| GSM8016850 |
PHYD-YFP/abCde, PHYB-GFP/AbCDE, PHYD-YFP/abcde mixture, 5 minutes, rep 1 |
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| Relations |
| BioProject |
PRJNA1064869 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE253236_RAW.tar |
90.3 Mb |
(http)(custom) |
TAR (of BW, NARROWPEAK) |
SRA Run Selector |
| Raw data are available in SRA |
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