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Series GSE253747 Query DataSets for GSE253747
Status Public on Apr 02, 2024
Title Aging aggravates aortic aneurysm and dissection via the miR-1204-MYLK signaling axis
Platform organisms Homo sapiens; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Macacine alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Herpesvirus saimiri (strain 11); JC polyomavirus; Human immunodeficiency virus 1; Human betaherpesvirus 6B; Human gammaherpesvirus 8; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The mechanism by which aging induces aortic aneurysm and dissection (AAD) remains unclear. A total of 430 subjects were recruited for screening of differentially expressed plasma microRNAs. We found that miR-1204 was significantly increased in both plasma and aorta of elder patients with AAD, and was positively correlated with age. Cell senescence induced the expression of miR-1204 through p53 interaction with plasmacytoma variant translocation 1, and miR-1204 induced vascular smooth muscle cell (VSMC) senescence to form a positive feedback loop. miR-1204 aggravated angiotensin II-induced AAD formation, and inhibition of miR-1204 attenuated β-aminopropionitrile monofumarate-induced AAD formation. Mechanistically, miR-1204 directly targeted myosin light chain kinase (MYLK) to promote VSMCs to acquire senescence-associated secretory phenotype (SASP) and lose their contractile phenotype. Overexpression of MYLK reversed miR-1204-induced VSMC senescence, SASP and contractile phenotype changes, and the decrease of transforming growth factor-β signaling pathway. Our findings suggest aging aggravates AAD via 75 miR-1204-MYLK signaling axis.
Overall design The plasma samples from young normal (< 50 years old), elder normal (> 50 years old), young patient (< 50 years old) and elder patient (> 50 years old) group (n=3 per group) were used for detecting the miRNA profiles using miRCURY LNA Array (version 19.0) (Exiqon miRCURY LNA™ microRNA Array, 7th gen-hsa, mmu and rno).
Contributor(s) Liu Z, Ou J
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Submission date Jan 19, 2024
Last update date Apr 03, 2024
Contact name Jingsong Ou
Phone 862087755766-8238
Organization name The First Affiliated Hospital, Sun Yat-sen University
Department Surgery/Cardiac Surgery
Street address 58 Zhong Shan Er Road
City Guangzhou
State/province Please Select
ZIP/Postal code 510080
Country China
Platforms (1)
GPL21439 miRCURY LNA microRNA Array, 7th generation - hsa, mmu & rno (miRBase 21; probeID version)
Samples (12)
GSM8027347 Young patient _rep1
GSM8027348 Young patient _rep2
GSM8027349 Young patient _rep3
BioProject PRJNA1066860

Download family Format
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Supplementary file Size Download File type/resource
GSE253747_All_Differentially_Expressed_miRNAs.xlsx 532.4 Kb (ftp)(http) XLSX
GSE253747_RAW.tar 11.5 Mb (http)(custom) TAR (of GPR)

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