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| Status |
Public on Apr 02, 2024 |
| Title |
Aging aggravates aortic aneurysm and dissection via the miR-1204-MYLK signaling axis |
| Platform organisms |
Homo sapiens; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Macacine alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Herpesvirus saimiri (strain 11); JC polyomavirus; Human immunodeficiency virus 1; Human betaherpesvirus 6B; Human gammaherpesvirus 8; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
The mechanism by which aging induces aortic aneurysm and dissection (AAD) remains unclear. A total of 430 subjects were recruited for screening of differentially expressed plasma microRNAs. We found that miR-1204 was significantly increased in both plasma and aorta of elder patients with AAD, and was positively correlated with age. Cell senescence induced the expression of miR-1204 through p53 interaction with plasmacytoma variant translocation 1, and miR-1204 induced vascular smooth muscle cell (VSMC) senescence to form a positive feedback loop. miR-1204 aggravated angiotensin II-induced AAD formation, and inhibition of miR-1204 attenuated β-aminopropionitrile monofumarate-induced AAD formation. Mechanistically, miR-1204 directly targeted myosin light chain kinase (MYLK) to promote VSMCs to acquire senescence-associated secretory phenotype (SASP) and lose their contractile phenotype. Overexpression of MYLK reversed miR-1204-induced VSMC senescence, SASP and contractile phenotype changes, and the decrease of transforming growth factor-β signaling pathway. Our findings suggest aging aggravates AAD via 75 miR-1204-MYLK signaling axis.
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| Overall design |
The plasma samples from young normal (< 50 years old), elder normal (> 50 years old), young patient (< 50 years old) and elder patient (> 50 years old) group (n=3 per group) were used for detecting the miRNA profiles using miRCURY LNA Array (version 19.0) (Exiqon miRCURY LNA™ microRNA Array, 7th gen-hsa, mmu and rno).
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| Contributor(s) |
Liu Z, Ou J |
| Citation missing |
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| Submission date |
Jan 19, 2024 |
| Last update date |
Apr 03, 2024 |
| Contact name |
Jingsong Ou |
| E-mail(s) |
oujs@mail.sysu.edu.cn
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| Phone |
862087755766-8238
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| Organization name |
The First Affiliated Hospital, Sun Yat-sen University
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| Department |
Surgery/Cardiac Surgery
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| Street address |
58 Zhong Shan Er Road
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| City |
Guangzhou |
| State/province |
Please Select |
| ZIP/Postal code |
510080 |
| Country |
China |
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| Platforms (1) |
| GPL21439 |
miRCURY LNA microRNA Array, 7th generation - hsa, mmu & rno (miRBase 21; probeID version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1066860 |
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