|
| Status |
Public on Apr 02, 2024 |
| Title |
Elder normal_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral Blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: plasma
gender: male
age: 60y
individual: Elder normal
|
| Treatment protocol |
Plasma was collected from blood anti-coagulated with ethylene diamine tetraaceticacid (EDTA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen) and miRNeasy mini kit (QIAGEN) according to manufacturer’s instructions, which efficiently recovered all RNA species, including miRNAs. RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis
|
| Label |
Hy3
|
| Label protocol |
The miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s guideline for miRNA labelling
|
| |
|
| Hybridization protocol |
The Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.16.0) (Exiqon) according to array manual
|
| Scan protocol |
The slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
|
| Description |
miRNA expression of human plasma
|
| Data processing |
Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=50 in all samples were chosen for calculating normalization factor. Expressed data were normalized using the Median normalization. After normalization, differentially expressed miRNAs with statistical significance were identified through Volcano Plot filtering
|
| |
|
| Submission date |
Jan 19, 2024 |
| Last update date |
Apr 02, 2024 |
| Contact name |
Jingsong Ou |
| E-mail(s) |
oujs@mail.sysu.edu.cn
|
| Phone |
862087755766-8238
|
| Organization name |
The First Affiliated Hospital, Sun Yat-sen University
|
| Department |
Surgery/Cardiac Surgery
|
| Street address |
58 Zhong Shan Er Road
|
| City |
Guangzhou |
| State/province |
Please Select |
| ZIP/Postal code |
510080 |
| Country |
China |
| |
|
| Platform ID |
GPL21439 |
| Series (1) |
| GSE253747 |
Aging aggravates aortic aneurysm and dissection via the miR-1204-MYLK signaling axis |
|
