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| Status |
Public on Apr 01, 2024 |
| Title |
Centromeric chromatin in mitosis reorganizes into a functional structure stabilized by cohesin [CENPA-ChIP-seq] |
| Organism |
Gallus gallus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Three-dimensional organization of mitotic chromosomes is established by cohesin and condensin complexes. In the centromere, cohesin maintains sister chromatid pairing until anaphase onset, while condensin provides elastic resistance to spindle forces. However, how condensin and cohesin structure vertebrate centromeres remains unclear. By super-resolution imaging, Capture-C analysis and polymer modeling we show that vertebrate centromeres are partitioned into two condensin-dependent subdomains during mitosis. This bipartite sub-structure is found in human, mouse and chicken centromeres, and is therefore a fundamental feature of vertebrate centromere. Super-resolution imaging and electron tomography reveal that bipartite centromeres assemble bipartite kinetochores with each subdomain capable of binding a distinct microtubule bundle. Cohesin helps to link the centromere subdomains, limiting their separation in response to spindle forces and preventing merotelic attachments. The two-domain structure described here may have implications for avoiding chromosomal instability as uncoupling of centromere subdomains is a common feature of lagging chromosomes in cancer cells.
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| Overall design |
Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for Histone veriant CENP-A to identify centromere region.
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| Contributor(s) |
Hori T, Samejima K, Webb S, Fukagawa T, Earnshaw WC |
| Citation missing |
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| |
| Submission date |
Jan 25, 2024 |
| Last update date |
Apr 01, 2024 |
| Contact name |
Shaun Michael Webb |
| E-mail(s) |
shaun.webb@ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
Wellcome Trust Centre for Cell Biology
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| Lab |
Bioinformatics Core Facility
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| Street address |
2.21 Swann Building, Kings Buildings
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| City |
Edinburgh |
| ZIP/Postal code |
EH9 3JR |
| Country |
United Kingdom |
| |
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| Platforms (2) |
| GPL19005 |
Illumina HiSeq 2500 (Gallus gallus) |
| GPL26853 |
Illumina NovaSeq 6000 (Gallus gallus) |
|
| Samples (8)
|
| GSM8035617 |
WT_CDK1as_subclone_1(biol rep1)_CENP-A_ChIP |
| GSM8035618 |
WT_CDK1as_subclone_1 (biol rep2)_CENP-A_ChIP |
| GSM8035619 |
SMC2-AID_CDK1as_subclone_1(biol rep1)_CENP-A_ChIP |
| GSM8035620 |
SMC2-AID_CDK1as_subclone_1 (biol rep2)_CENP-A_ChIP |
| GSM8035621 |
SMC3-AID_CDK1as_subclone_1(biol rep1)_CENP-A_ChIP |
| GSM8035622 |
SMC3-AID_CDK1as_subclone_1 (biol rep2)_CENP-A_ChIP |
| GSM8035623 |
CAP-H_Halo_CDK1as(biol rep1)_CENP-A_ChIP |
| GSM8035624 |
Halo-CAP-H2_CDK1as(biol rep1)_CENP-A_ChIP |
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| This SubSeries is part of SuperSeries: |
| GSE254182 |
Centromeric chromatin in mitosis reorganizes into a functional structure stabilized by cohesin |
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| Relations |
| BioProject |
PRJNA1068948 |
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