|
| Status |
Public on Dec 10, 2010 |
| Title |
AdultMale-H3K27me3 |
| Project |
modENCODE
|
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
modENCODE_submission_372
This submission comes from a modENCODE project of Kevin White. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: The White Lab is aiming to map the association of the main post-translationally modified histones on the genome of Drosophila melanogaster. One technique that we use for this purpose is chromatin immunoprecipitation-on-chip (ChIP-on-chip) utilizing whole-genome tiling arrays. The data generated by ChIP-chip experiments consist basically of a plot of signal intensity across the genome. The highest signals correspond to positions in the genome occupied by the tested histone modification.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
CHIP-chip
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| |
|
| Overall design |
EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: Y cn bw sp(genotype : y[1] oc[R3.2]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] lab[R4.2] MstProx[1] GstD5[1] Rh6[1] official name : y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1] ); Developmental Stage: Adult Male; Genotype: y[1] oc[R3.2]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] lab[R4.2] MstProx[1] GstD5[1] Rh6[1]; Sex: Male; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Developmental Stage Adult Male; Antibody H3K27me3 (target is H3K27me3); Strain Y cn bw sp(genotype : y[1] oc[R3.2]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] lab[R4.2] MstProx[1] GstD5[1] Rh6[1] official name : y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1] )
|
| Web link |
http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
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| |
|
| Contributor(s) |
White K, Negre N, Shah P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA63467 |
| |
| Submission date |
Dec 09, 2010 |
| Last update date |
Aug 19, 2013 |
| Contact name |
DCC modENCODE |
| E-mail(s) |
help@modencode.org
|
| Phone |
416-673-8579
|
| Organization name |
Ontario Institute for Cancer Research
|
| Lab |
modENCODE DCC
|
| Street address |
MaRS Centre, South Tower, 101 College Street, Suite 800
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
| |
|
| Platforms (3) |
| GPL6949 |
Agilent-019182 Drosophila melanogaster Whole Genome ChIP-on-Chip Set 244K, Microarray 1 of 3 |
| GPL6950 |
Agilent-019183 Drosophila melanogaster Whole Genome ChIP-on-Chip Set 244K, Microarray 2 of 3 |
| GPL6951 |
Agilent-019184 Drosophila melanogaster Whole Genome ChIP-on-Chip Set 244K, Microarray 3 of 3 |
|
| Samples (9)
|
| GSM637602 |
Male-K27Me3_2 extraction2_array1 |
| GSM637603 |
Male-K27Me3_2 extraction2_array2 |
| GSM637604 |
Male-K27Me3_2 extraction2_array3 |
| GSM637605 |
AdultMale-K27Me3_3 extraction3_array1 |
| GSM637606 |
AdultMale-K27Me3_3 extraction3_array2 |
| GSM637607 |
AdultMale-K27Me3_3 extraction3_array3 |
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