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Series GSE25973 Query DataSets for GSE25973
Status Public on Mar 03, 2011
Title FGF2 sustains NANOG and switches the outcome of BMP4 induced human embryonic stem cell differentiation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary NANOG has emerged as a central gatekeeper of pluripotency. Here we show that as human embryonic stem (ES) cells exit the pluripotent state, NANOG can play a key role in determining lineage outcome. It has previously been reported that BMPs can induce differentiation of human ES cells into extraembryonic lineages. Here we report that FGF2 switches BMP4 induced differentiation outcome to mesendoderm, characterized by the uniform expression of T (brachyury) and other primitive streak markers. Blocking the MEK-ERK pathway either by chemical inhibitors or by an ERK-specific phosphatase (DUSP6) blocks the FGF2-mediated lineage switch. Active MEK-ERK signaling prolongs NANOG expression during BMP-induced differentiation. Forced NANOG expression results in FGF independent BMP4 induction of mesendoderm, and knockdown of NANOG greatly reduces T induction. Together, our results demonstrate that FGF2 signaling switches the outcome of BMP4 induced differentiation of human ES cells by maintaining NANOG levels through the MEK-ERK pathway.
 
Overall design There are three sets of expression data. Set 1 (14 samples) is 5day human ES cells (H1) differentiated with different concentrations of BMP4, in the presence or absence of FGF2. Set 2 (14 samples) is 50ng/mL of BMP4 induced H1 cells differentiation time course, with or without FGF2. Set 3 (22 samples) is 5ng/mL of BMP4 induced H1 cells differentiation time course, with or without FGF2.
 
Contributor(s) Yu P, Stewart R, Tian S, Thomson J
Citation(s) 21362572
Submission date Dec 09, 2010
Last update date Mar 22, 2012
Contact name James Thomson
E-mail(s) JThomson@Morgridgeinstitute.org
Phone 608-263-3585
Fax 608-265-8984
Organization name Mogridge Institute for Research
Department Regenerative Biology
Street address 425 Henry Mall
City madison
State/province Wisconsin
ZIP/Postal code 53706
Country USA
 
Platforms (2)
GPL6602 HG17_Human_60mer_1in2
GPL9999 NimbleGen human hg18 array (2007-06-15_WiCell_HG18_p14)
Samples (50)
GSM637810 H1 control in TeSR1 (Control_102540)
GSM637811 3ng/mL BMP4 + FGF2 (3ng_103248)
GSM637812 6ng/mL BMP4 + FGF2 (6ng_102545)
Relations
BioProject PRJNA135449

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25973_RAW.tar 736.2 Mb (http)(custom) TAR (of PAIR)
Processed data included within Sample table
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