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| Status |
Public on Jun 18, 2024 |
| Title |
Epigenetic and transcriptional consequences of chemically induced transposon mobilization in the endosperm (CUT&Tag) |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Genomic imprinting, an epigenetic phenomenon leading to parent-of-origin-specific gene expression, has independently evolved in the endosperm of flowering plants and the placenta of mammals—tissues crucial for nurturing embryos. While transposable elements (TEs) frequently colocalize with imprinted genes and are implicated in imprinting establishment, direct investigations of the impact of de novo TE transposition on genomic imprinting remain scarce. In this study, we explored the effects of chemically induced transposition of the Copia element ONSEN on genomic imprinting in Arabidopsis thaliana. Through the combination of chemical TE mobilization and doubled haploid induction, we generated a line with 40 new ONSEN copies. Our findings reveal a preferential targeting of maternally expressed genes (MEGs) for transposition, aligning with the colocalization of H2A.Z and H3K27me3 in MEGs—both previously identified as promoters of ONSEN insertions. Additionally, we demonstrate that chemically-induced DNA hypomethylation induces global transcriptional deregulation in the endosperm, leading to the breakdown of MEG imprinting. This study provides insights into the consequences of chemically induced TE remobilization in the endosperm, underscoring the need for cautious interpretation of the connection between TEs and genomic imprinting.
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| Overall design |
RNAseq, WGBS and CUT&Tag datasets were obtained from INTACT purified endosperm nuclei from Col × Ler (L) reciprocal crosses at 4 days after pollination (DAP). WGBS and CUT&Tag were generated in duplicates using the INTACT line (referred as C) or TEmob (T3, referred as Cm) genetic background in recirpocal crosses with Ler (L). RNAseq libraries were generated in triplicates from TEmob (Cm) x Ler (L) reciprocal crosses.
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| Contributor(s) |
del Toro de Leon G, van Boven J, Santos-González J, Wen-Biao J, Schneeberger K, Köhler C |
| Citation(s) |
38967011 |
| |
| Submission date |
Mar 04, 2024 |
| Last update date |
Sep 17, 2024 |
| Contact name |
Juan C Santos-González |
| E-mail(s) |
juan.santos@slu.se
|
| Organization name |
SLU
|
| Department |
Department of Plant Biology and Forest Genetics
|
| Lab |
Köhler's lab
|
| Street address |
Almas Allé 5
|
| City |
Uppsala |
| State/province |
Uppsala |
| ZIP/Postal code |
75007 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL34120 |
NextSeq 1000 (Arabidopsis thaliana) |
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| Samples (24)
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| GSM8125020 |
at-endosperm-H3K9m2_replicate1_col_C_x_ler |
| GSM8125021 |
at-endosperm-H3_replicate1_col_C_x_ler |
| GSM8125022 |
at-endosperm-H3K27m3_replicate1_col_C_x_ler |
| GSM8125023 |
at-endosperm-H3K9m2_replicate1_ler_x_col_C |
| GSM8125024 |
at-endosperm-H3_replicate1_ler_x_col_C |
| GSM8125025 |
at-endosperm-H3K27m3_replicate1_ler_x_col_C |
| GSM8125026 |
at-endosperm-H3K9m2_replicate2_col_C_x_ler |
| GSM8125027 |
at-endosperm-H3_replicate2_col_C_x_ler |
| GSM8125028 |
at-endosperm-H3K27m3_replicate2_col_C_x_ler |
| GSM8125029 |
at-endosperm-H3K9m2_replicate2_ler_x_col_C |
| GSM8125030 |
at-endosperm-H3_replicate2_ler_x_col_C |
| GSM8125031 |
at-endosperm-H3K27m3_replicate2_ler_x_col_C |
| GSM8125032 |
at-endosperm-H3K9m2_replicate1_col_Cm_x_ler |
| GSM8125033 |
at-endosperm-H3_replicate1_col_Cm_x_ler |
| GSM8125034 |
at-endosperm-H3K27m3_replicate1_col_Cm_x_ler |
| GSM8125035 |
at-endosperm-H3K9m2_replicate1_ler_x_col_Cm |
| GSM8125036 |
at-endosperm-H3_replicate1_ler_x_col_Cm |
| GSM8125037 |
at-endosperm-H3K27m3_replicate1_ler_x_col_Cm |
| GSM8125038 |
at-endosperm-H3K9m2_replicate2_col_Cm_x_ler |
| GSM8125039 |
at-endosperm-H3_replicate2_col_Cm_x_ler |
| GSM8125040 |
at-endosperm-H3K27m3_replicate2_col_Cm_x_ler |
| GSM8125041 |
at-endosperm-H3K9m2_replicate2_ler_x_col_Cm |
| GSM8125042 |
at-endosperm-H3_replicate2_ler_x_col_Cm |
| GSM8125043 |
at-endosperm-H3K27m3_replicate2_ler_x_col_Cm |
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| This SubSeries is part of SuperSeries: |
| GSE260822 |
Epigenetic and transcriptional consequences in the endosperm of chemically induced transposon mobilization in Arabidopsis |
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| Relations |
| BioProject |
PRJNA1083574 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE260818_h3_CmxL_mx_COL_RPGC_bs50.bw.gz |
8.1 Mb |
(ftp)(http) |
BW |
| GSE260818_h3_CmxL_mx_LER_RPGC_bs50.bw.gz |
4.9 Mb |
(ftp)(http) |
BW |
| GSE260818_h3_CxL_mx_COL_RPGC_bs50.bw.gz |
8.7 Mb |
(ftp)(http) |
BW |
| GSE260818_h3_CxL_mx_LER_RPGC_bs50.bw.gz |
6.7 Mb |
(ftp)(http) |
BW |
| GSE260818_h3_LxC_mx_COL_RPGC_bs50.bw.gz |
7.1 Mb |
(ftp)(http) |
BW |
| GSE260818_h3_LxC_mx_LER_RPGC_bs50.bw.gz |
8.3 Mb |
(ftp)(http) |
BW |
| GSE260818_h3_LxCm_mx_COL_RPGC_bs50.bw.gz |
5.8 Mb |
(ftp)(http) |
BW |
| GSE260818_h3_LxCm_mx_LER_RPGC_bs50.bw.gz |
7.5 Mb |
(ftp)(http) |
BW |
| GSE260818_k27_CmxL_mx_COL_RPGC_bs50.bw.gz |
8.0 Mb |
(ftp)(http) |
BW |
| GSE260818_k27_CmxL_mx_LER_RPGC_bs50.bw.gz |
3.7 Mb |
(ftp)(http) |
BW |
| GSE260818_k27_CxL_mx_COL_RPGC_bs50.bw.gz |
8.6 Mb |
(ftp)(http) |
BW |
| GSE260818_k27_CxL_mx_LER_RPGC_bs50.bw.gz |
4.9 Mb |
(ftp)(http) |
BW |
| GSE260818_k27_LxC_mx_COL_RPGC_bs50.bw.gz |
5.0 Mb |
(ftp)(http) |
BW |
| GSE260818_k27_LxC_mx_LER_RPGC_bs50.bw.gz |
8.2 Mb |
(ftp)(http) |
BW |
| GSE260818_k27_LxCm_mx_COL_RPGC_bs50.bw.gz |
4.7 Mb |
(ftp)(http) |
BW |
| GSE260818_k27_LxCm_mx_LER_RPGC_bs50.bw.gz |
7.9 Mb |
(ftp)(http) |
BW |
| GSE260818_k9_CmxL_mx_COL_RPGC_bs50.bw.gz |
6.9 Mb |
(ftp)(http) |
BW |
| GSE260818_k9_CmxL_mx_LER_RPGC_bs50.bw.gz |
3.5 Mb |
(ftp)(http) |
BW |
| GSE260818_k9_CxL_mx_COL_RPGC_bs50.bw.gz |
5.9 Mb |
(ftp)(http) |
BW |
| GSE260818_k9_CxL_mx_LER_RPGC_bs50.bw.gz |
3.2 Mb |
(ftp)(http) |
BW |
| GSE260818_k9_LxC_mx_COL_RPGC_bs50.bw.gz |
4.2 Mb |
(ftp)(http) |
BW |
| GSE260818_k9_LxC_mx_LER_RPGC_bs50.bw.gz |
6.0 Mb |
(ftp)(http) |
BW |
| GSE260818_k9_LxCm_mx_COL_RPGC_bs50.bw.gz |
3.4 Mb |
(ftp)(http) |
BW |
| GSE260818_k9_LxCm_mx_LER_RPGC_bs50.bw.gz |
5.1 Mb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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