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| Status |
Public on Jan 02, 2006 |
| Title |
Whole Transcriptome Amplification for Gene Expression Profiling and Development of Molecular Archives |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Type of experiment: This study used microarray experiments to evaluate the reproducibility and fidelity of Whole Transcriptome Amplification (WTA).
Experimental factors: This study contains multiple categories of microarray experiments. Self-self hybridizations from WTA amplified total RNA were performed to evaluate the reproducibility of WTA. To evaluate the fidelity of WTA, the same total RNA, isolated from tissue samples or cell lines, was either directly used for microarray analysis or subjected to WTA amplification before hybridization. The amount of input total RNA for WTA amplification was varied for different hybridizations. To demonstrate the usefulness of WTA, benign epithelia, cancerous epithelia and stroma isolated by laser capture microdissection from frozen prostate tissue specimens were subjected to WTA and hybridized. To demonstrate the fidelity of WTA with degraded samples, total RNA from cell lines was artificially degraded before WTA and hybridization. Total RNA isolated from formalin-fixed paraffin-embedded (FFPE) tissues containing benign and cancerous prostate tissue were also subjected to WTA and hybridized.
Keywords: other
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| Contributor(s) |
Tomlins SA, Mehra R, Rhodes DR, Shah RB, Rubin MA, Bruening E, Makarov V, Chinnaiyan AM |
| Citation(s) |
16611408 |
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| Submission date |
May 04, 2005 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Scott Tomlins |
| E-mail(s) |
tomlinss@med.umich.edu
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| Phone |
734-615-1417
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| Organization name |
University of Michigan
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| Department |
Pathology
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| Lab |
Chinnaiyan Lab
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| Street address |
1400 E. Medical Center Dr., 5410 CCGC
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (2) |
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| Samples (41)
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| Relations |
| BioProject |
PRJNA92083 |
| Supplementary data files not provided |
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