Data table header descriptions |
ID_REF |
|
VALUE |
Normalized (lowess window 0.6), log base 2 |
X |
the X-coordinate in µm of the center of the feature-indicator associated with the feature, where (0,0) is the top left of the image. |
Y |
the Y-coordinate in µm of the center of the feature-indicator associated with the feature, where (0,0) is the top left of the image. |
Dia. |
the diameter in µm of the feature-indicator. |
F635 Median |
median feature pixel intensity at wavelength #1 (635 nm) (Cy5). |
F635 Mean |
mean feature pixel intensity at wavelength #1 (635 nm). |
F635 SD |
the standard deviation of the feature pixel intensity at wavelength #1 (635 nm). |
B635 Median |
the median feature background intensity at wavelength #1 (635 nm). |
B635 Mean |
the mean feature background intensity at wavelength #1 (635 nm). |
B635 SD |
the standard deviation of the feature background intensity at wavelength #1 (635 nm) |
% > B635+1SD |
the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #1 (635 nm). |
% > B635+2SD |
the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #1 (635 nm). |
F635 % Sat. |
the percentage of feature pixels at wavelength #1 that are saturated. |
F532 Median |
median feature pixel intensity at wavelength #2 (532 nm) (Cy3). |
F532 Mean |
mean feature pixel intensity at wavelength #2 (532 nm). |
F532 SD |
the standard deviation of the feature intensity at wavelength #2 (532 nm). |
B532 Median |
the median feature background intensity at wavelength #2 (532 nm). |
B532 Mean |
the mean feature background intensity at wavelength #2 (532 nm). |
B532 SD |
the standard deviation of the feature background intensity at wavelength #2 (532 nm). |
% > B532+1SD |
the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #2 (532 nm). |
% > B532+2SD |
the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #2 (532 nm). |
F532 % Sat. |
the percentage of feature pixels at wavelength #2 that are saturated. |
Ratio of Medians (635/532) |
the ratio of the median intensities of each feature for each wavelength, with the median background subtracted. |
Ratio of Means (635/532) |
the ratio of the arithmetic mean intensities of each feature for each wavelength, with the median background subtracted. |
Median of Ratios (635/532) |
the median of pixel-by-pixel ratios of pixel intensities, with the median background subtracted. |
Mean of Ratios (635/532) |
the arithmetic mean of the pixel-by-pixel ratios of pixel intensities, with the median background subtracted. |
Ratios SD (635/532) |
the standard deviation of pixel intensity ratios. |
Rgn Ratio (635/532) |
the regression ratio. |
Rgn R? |
the coefficient of determination for the current regression value. |
F Pixels |
the total number of feature pixels. |
B Pixels |
the total number of background pixels |
Sum of Medians |
the sum of the median intensities for each wavelength, with the median background subtracted. |
Sum of Means |
the sum of the arithmetic mean intensities for each wavelength, with the median background subtracted. |
Log Ratio (635/532) |
log (base 2) transform of the ratio of the medians. |
F635 Median - B635 |
the median feature pixel intensity at wavelength #1 with the median background subtracted. |
F532 Median - B532 |
the median feature pixel intensity at wavelength #2 with the median background subtracted. |
F635 Mean - B635 |
the mean feature pixel intensity at wavelength #1 with the median background subtracted. |
F532 Mean - B532 |
the mean feature pixel intensity at wavelength #2 with the median background subtracted. |
Flags |
the type of flag associated with a feature. Features that could not be identified by Genepix are indicated with -50. Empty spots are indicated with -75. |