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| Status |
Public on Mar 26, 2024 |
| Title |
Chromatin and gene expression profile at single nuclei level of CD45+ leukocytes from the rhesus brain parenchyma |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We used Chromium Single Cell Multiome ATAC + Gene Expression to analyze CD45+ leukocytes in the rhesus macaque brain during homeostasis and chronic SIV infection
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| Overall design |
Leukocytes (CD45+ cells) of previously cryopreserved rhesus macaque brain single cell suspensions were isolated using MACS CD45 microbeads/column purification followed by Fluorescence-activated cell sorting (FACS) according to the presence of absence of CD45 signal. Nuclei were extracted according to 10x protocols and analyzed using single snATACseq
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| Contributor(s) |
Elizaldi S, Iyer S, Li J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 19, 2024 |
| Last update date |
Mar 26, 2024 |
| Contact name |
Sonny Ramirez Elizaldi |
| E-mail(s) |
Srelizaldi@ucdavis.edu
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| Organization name |
University of California Davis
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| Department |
Immunology
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| Lab |
Iyer Lab
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| Street address |
County Road 98 and, Hutchison Dr
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| City |
Woodland |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (1) |
| GPL27943 |
Illumina NovaSeq 6000 (Macaca mulatta) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1089650 |
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