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| Status |
Public on Apr 05, 2024 |
| Title |
Comprehensive analysis of circular RNA profiling in AZD9291-resistant non-small cell lung cancer cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Considering the important role of circular RNAs (circRNAs) in cancers, we established AZD9291-resistant NSCLC cell lines (H1975/AZDR and HCC827/AZDR) and used microarray analysis to determine the circRNA expression profiles of the cells. The H1975/AZDR and HCC827/AZDR cell lines were induced by gradually increasing the drug concentration. CircRNA microarray expression profiles were obtained from H1975, HCC827, H1975/AZDR, and HCC827/AZDR cells and validated by quantitative reverse transcription PCR. Expression data were analyzed bioinformatically.
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| Overall design |
We first established AZD9291-resistant H1975 and HCC827 cell lines (H1975/AZDR and HCC827/AZDR) by gradually increasing concentrations of AZD9291 for 72 hours with a recovery period between treatments.The newly established H1975/AZDR and HCC827/AZDR cell lines were maintained in a medium containing 10μM AZD9291, while the parental H1975 and HCC827 cell lines were cultured in drug-free medium.
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| Contributor(s) |
Yang Y, Luo Q, Chen T, Luo J, Gu Y, Huang J |
| Citation missing |
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| Submission date |
Apr 02, 2024 |
| Last update date |
Apr 05, 2024 |
| Contact name |
Tianxiang Chen |
| E-mail(s) |
txchen@shsmu.edu.cn
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| Organization name |
Shanghai Chest Hospital
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| Street address |
Shanghai Chest hospital
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| City |
Shanghai |
| ZIP/Postal code |
200052 |
| Country |
China |
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| Platforms (1) |
| GPL22120 |
Agilent-078298 human ceRNA array V1.0 4X180K [Probe Name Version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1095434 |
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